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Label free monitoring of megakaryocytic development and proplatelet formation in vitro.
Pouli, Dimitra; Tozzi, Lorenzo; Alonzo, Carlo A; Liu, Zhiyi; Kaplan, David L; Balduini, Alessandra; Georgakoudi, Irene.
Afiliación
  • Pouli D; Department of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
  • Tozzi L; These authors contributed equally to this work.
  • Alonzo CA; Department of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
  • Liu Z; Department of Molecular Medicine, University of Pavia, Pavia, Italy.
  • Kaplan DL; Biotechnology Research Laboratories, IRCCS San Matteo Foundation, Pavia, Italy.
  • Balduini A; These authors contributed equally to this work.
  • Georgakoudi I; Department of Biomedical Engineering, Tufts University, 4 Colby St., 02155 Medford MA, USA.
Biomed Opt Express ; 8(10): 4742-4755, 2017 Oct 01.
Article en En | MEDLINE | ID: mdl-29082099
Megakaryopoiesis and platelet production are complex biological processes that require tight regulation of successive lineage commitment steps and are ultimately responsible for maintaining and renewing the pool of circulating platelets in the blood. Despite major advancements in the understanding of megakaryocytic biology, the detailed mechanisms driving megakaryocytic differentiation have yet to be elucidated. Here we show that automated image analysis algorithms applied to two-photon excited fluorescence (TPEF) images can non-invasively monitor structural and metabolic megakaryocyte behavior changes occurring during differentiation and platelet formation in vitro. Our results demonstrate that high-contrast, label-free two photon imaging holds great potential in studying the underlying physiological processes controlling the intricate process of platelet production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos