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FRET Between Riboflavin and 9-Anthraldehyde Based Fluorescent Organic Nanoparticles Possessing Antibacterial Activity.
Mahajan, Prasad G; Dige, Nilam C; Suryawanshi, Sonali B; Dalavi, Dattatray K; Kamble, Avinash A; Bhopate, Dhanaji P; Kadam, Abhijit N; Kondalkar, Vijay V; Kolekar, Govind B; Patil, Shivajirao R.
Afiliación
  • Mahajan PG; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India. mahajanprasad2188@gmail.com.
  • Dige NC; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India.
  • Suryawanshi SB; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India.
  • Dalavi DK; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India.
  • Kamble AA; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India.
  • Bhopate DP; Division of Advanced Materials Engineering, Chonbuk National University, Jeonju, 561-756, South Korea.
  • Kadam AN; Department of Chemical and Biochemical Engineering, Gachon University, Seongnam, 461-701, South Korea.
  • Kondalkar VV; Department of Electrical and Computer Engineering, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon, 443-749, South Korea.
  • Kolekar GB; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India.
  • Patil SR; Department of Chemistry, Shivaji University, Kolhapur, 416004, Maharashtra, India. srp_fsl@rediffmail.com.
J Fluoresc ; 28(1): 207-215, 2018 Jan.
Article en En | MEDLINE | ID: mdl-29079896
The aqueous suspension of fluorescent nanoparticles were prepared by using 9-anthradehdye derivative (AH). The nanoparticles (AHNPs) were characterized using DLS-zeta sizer and SEM techniques. The photo physical properties of nanoparticles and precursor were measured and compared using UV-absorption spectroscopy, fluorescence spectroscopy and fluorescence lifetime studies. The significant overlap between fluorescence spectrum of AHNPs and excitation spectrum of Riboflavin (RF) led us to explore Fluorescence Resonance Energy Transfer (FRET) studies between AHNPs and RF in aqueous medium. The mechanism of FRET from AHNPs to RF discussed on spectral observations, thermodynamic parameters and changes produces in fluorescence lifetime in absence and presence of different concentrations of RF to AHNPs. The limit of detection for RF (0.071 µM) is considerably low compared with reported methods. Thus, we explore AHNPs as novel nano probe for quantitative determination of RF in pharmaceutical samples based on FRET study. In addition with this, AHNPs has excellent antibacterial activity than the bulk material for two different bacteria culture viz. E. coli and Bacillus sps. Graphical Abstract 9-anthradehdye based fluorescent nanoparticles (AHNPs) explores as nano probe to detect Riboflavin (RF) in aqueous medium based on Fluorescence Resonance Energy Transfer (FRET) studies. The proposed analytical method successfully applied for quantitative determination of RF in pharmaceutical samples. In addition, with this, AHNPs has excellent antibacterial activity than the bulk material for two different bacteria culture suspension viz. E. coli and Bacillus sps.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Riboflavina / Bacillus / Escherichia coli / Nanopartículas / Colorantes Fluorescentes / Antracenos / Antibacterianos Idioma: En Revista: J Fluoresc Asunto de la revista: BIOFISICA Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Riboflavina / Bacillus / Escherichia coli / Nanopartículas / Colorantes Fluorescentes / Antracenos / Antibacterianos Idioma: En Revista: J Fluoresc Asunto de la revista: BIOFISICA Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos