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Male fertility restored by transplanting primordial germ cells into testes: a new way towards efficient transgenesis in chicken.
Trefil, Pavel; Aumann, Dorothea; Koslová, Anna; Mucksová, Jitka; Benesová, Barbora; Kalina, Jirí; Wurmser, Christine; Fries, Ruedi; Elleder, Daniel; Schusser, Benjamin; Hejnar, Jirí.
Afiliación
  • Trefil P; BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 254 49, Jilove u Prahy, Czech Republic.
  • Aumann D; Reproductive Biotechnology, Technical University Munich, Liesel-Beckmann Street 1/III, 85354, Freising, Germany.
  • Koslová A; Institute of Molecular Genetics of the Czech Academy of Sciences, Videnska 1083, 14220, Prague, Czech Republic.
  • Mucksová J; BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 254 49, Jilove u Prahy, Czech Republic.
  • Benesová B; BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 254 49, Jilove u Prahy, Czech Republic.
  • Kalina J; BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 254 49, Jilove u Prahy, Czech Republic.
  • Wurmser C; Chair of Animal Breeding, Technical University Munich, Liesel-Beckmann Street 1/III, 85354, Freising, Germany.
  • Fries R; Chair of Animal Breeding, Technical University Munich, Liesel-Beckmann Street 1/III, 85354, Freising, Germany.
  • Elleder D; Institute of Molecular Genetics of the Czech Academy of Sciences, Videnska 1083, 14220, Prague, Czech Republic.
  • Schusser B; Reproductive Biotechnology, Technical University Munich, Liesel-Beckmann Street 1/III, 85354, Freising, Germany. benjamin.schusser@tum.de.
  • Hejnar J; Institute of Molecular Genetics of the Czech Academy of Sciences, Videnska 1083, 14220, Prague, Czech Republic. hejnar@img.cas.cz.
Sci Rep ; 7(1): 14246, 2017 10 27.
Article en En | MEDLINE | ID: mdl-29079843
The ongoing progress in primordial germ cell derivation and cultivation is opening new ways in reproductive biotechnology. This study tested whether functional sperm cells can be matured from genetically manipulated primordial germ cells after transplantation in adult testes and used to restore fertility. We show that spermatogenesis can be restored after mCherry-expressing or GFP-expressing primordial germ cells are transplantated into the testes of sterilized G0 roosters and that mCherry-positive or GFP-positive non-chimeric transgenic G1 offspring can be efficiently produced. Compared with the existing approaches to primordial germ cell replacement, this new technique eliminates the germ line chimerism of G0 roosters and is, therefore, faster, more efficient and requires fewer animals. Furthermore, this is the only animal model, where the fate of primordial germ cells in infertile recipients can be studied.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Testículo / Pollos / Técnicas de Transferencia de Gen / Trasplante de Células / Fertilidad Límite: Animals Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Testículo / Pollos / Técnicas de Transferencia de Gen / Trasplante de Células / Fertilidad Límite: Animals Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido