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MARK1 is a Novel Target for miR-125a-5p: Implications for Cell Migration in Cervical Tumor Cells.
Natalia, Martinez-Acuna; Alejandro, Gonzalez-Torres; Virginia, Tapia-Vieyra Juana; Alvarez-Salas, Luis Marat.
Afiliación
  • Natalia MA; Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del I.P.N., Ciudad de Mexico, Mexico.
  • Alejandro GT; Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del I.P.N., Ciudad de Mexico, Mexico.
  • Virginia TJ; Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del I.P.N., Ciudad de Mexico, Mexico.
  • Alvarez-Salas LM; Laboratorio de Terapia Genica, Departamento de Genetica y Biologia Molecular, Centro de Investigacion y de Estudios Avanzados del I.P.N., Ciudad de Mexico, Mexico.
Microrna ; 7(1): 54-61, 2018.
Article en En | MEDLINE | ID: mdl-29076440
BACKGROUND: Aberrant miRNA expression is associated with the development of several diseases including cervical cancer. Dysregulation of miR-125a-5p is present in a plethora of tumors, but its role in cervical cancer is not well understood. OBJECTIVE: The aim was to analyze the expression profile of miR-125a-5p in tumor and immortal cell lines with further target prediction, validation and function analysis. METHODS: MiR-125a-5p expression was determined by real-time RT-PCR from nine cervical cell lines. In silico tools were used to find target transcripts with an miR-125-5p complementary site within the 3'UTR region. Further target selection was based on gene ontology annotation and ΔG analysis. Target validation was performed by transfection of synthetic miR-125a-5p mimics and luciferase assays. Functional evaluation of miR-125a-5p on migration was performed by transwell migration assays. RESULTS: Differential miR-125a-5p expression was observed between immortal and tumor cells regardless of the human papillomavirus (HPV) content. Thermodynamic and ontological analyses showed Microtubule-Affinity-Regulating Kinase1 (MARK1) as a putative target for miR-125a-5p. An inverse correlation was observed among miR-125a-5p expression and MARK1 protein levels in tumor but not in immortal cells. Luciferase assays showed direct miR-125a-5p regulation over MARK1 through recognition of a predicted target site within the 3'-UTR. HeLa and C-33A cervical tumor cells enhanced migration after transfection with miR-125a-5p mimics and stimulation of cell migration was reproduced by siRNA-mediated inhibition of MARK1. CONCLUSION: The results showed MARK1 as a novel functional target for miR-125a-5p with implications on cell migration of tumor cervical cancer cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / Movimiento Celular / Neoplasias del Cuello Uterino / Proteínas Serina-Treonina Quinasas / MicroARNs Límite: Female / Humans Idioma: En Revista: Microrna Año: 2018 Tipo del documento: Article País de afiliación: México Pais de publicación: Emiratos Árabes Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / Movimiento Celular / Neoplasias del Cuello Uterino / Proteínas Serina-Treonina Quinasas / MicroARNs Límite: Female / Humans Idioma: En Revista: Microrna Año: 2018 Tipo del documento: Article País de afiliación: México Pais de publicación: Emiratos Árabes Unidos