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Unveiling the stimulatory effects of tartrazine on human and bovine serum albumin fibrillogenesis: Spectroscopic and microscopic study.
Al-Shabib, Nasser Abdulatif; Khan, Javed Masood; Alsenaidy, Mohammad A; Alsenaidy, Abdulrahman M; Khan, Mohd Shahnawaz; Husain, Fohad Mabood; Khan, Mohammad Rashid; Naseem, Mohammad; Sen, Priyankar; Alam, Parvez; Khan, Rizwan Hasan.
Afiliación
  • Al-Shabib NA; Department of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University, 2460, Riyadh 11451, Saudi Arabia. Electronic address: nalshabib@ksu.edu.sa.
  • Khan JM; Department of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University, 2460, Riyadh 11451, Saudi Arabia.
  • Alsenaidy MA; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • Alsenaidy AM; Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
  • Khan MS; Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
  • Husain FM; Department of Food Science and Nutrition, Faculty of Food and Agricultural Sciences, King Saud University, 2460, Riyadh 11451, Saudi Arabia.
  • Khan MR; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • Naseem M; Electrical Engineering Department, Integral University Lucknow, 226026, India.
  • Sen P; Centre for Bioseparation Technology, VIT University, Vellore 632014, India.
  • Alam P; Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
  • Khan RH; Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India.
Spectrochim Acta A Mol Biomol Spectrosc ; 191: 116-124, 2018 Feb 15.
Article en En | MEDLINE | ID: mdl-29028503
Amyloid fibrils are playing key role in the pathogenesis of various neurodegenerative diseases. Generally anionic molecules are known to induce amyloid fibril in several proteins. In this work, we have studied the effect of anionic food additive dye i.e., tartrazine (TZ) on the amyloid fibril formation of human serum albumins (HSA) and bovine serum albumin (BSA) at pHs7.4 and 3.5. We have employed various biophysical methods like, turbidity measurements, Rayleigh Light Scattering (RLS), Dynamic Light Scattering (DLS), intrinsic fluorescence, Congo red assay, far-UV CD, transmission electron microscopy (TEM) and atomic force microscopy (AFM) to decipher the mechanism of TZ-induce amyloid fibril formation in both the serum albumins at pHs7.4 and 3.5. The obtained results suggest that both the albumins forms amyloid-like aggregates in the presence of 1.0 to 15.0mM of TZ at pH3.5, but no amyloid fibril were seen at pH7.4. The possible cause of TZ-induced amyloid fibril formation is electrostatic and hydrophobic interaction because sulfate group of TZ may have interacted electrostatically with positively charged amino acids of the albumins at pH3.5 and increased protein-protein and protein-TZ interactions leading to amyloid fibril formation. The TEM, RLS and DLS results are suggesting that BSA forms bigger size amyloids compared to HSA, may be due to high surface hydrophobicity of BSA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tartrazina / Albúmina Sérica Bovina / Albúmina Sérica Humana Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tartrazina / Albúmina Sérica Bovina / Albúmina Sérica Humana Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article Pais de publicación: Reino Unido