Your browser doesn't support javascript.
loading
Coadministration of Recombinant Adenovirus Expressing GM-CSF with Inactivated H5N1 Avian Influenza Vaccine Increased the Immune Responses and Protective Efficacy Against a Wild Bird Source of H5N1 Challenge.
Wang, Xiangwei; Wang, Xinglong; Jia, Yanqing; Wang, Chongyang; Tang, Qiuxia; Han, Qingsong; Xiao, Sa; Yang, Zengqi.
Afiliación
  • Wang X; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Wang X; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Jia Y; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Wang C; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Tang Q; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Han Q; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Xiao S; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
  • Yang Z; Department of Avian Disease, College of Veterinary Medicine, Northwest A&F University , Yangling, People's Republic of China .
J Interferon Cytokine Res ; 37(10): 467-473, 2017 10.
Article en En | MEDLINE | ID: mdl-29028432
Wild birds play a key role in the spread of avian influenza virus (AIV). There is a continual urgent requirement for AIV vaccines to address the ongoing genetic changes of AIV. In the current study, we trialed a novel AIV vaccine against the wild bird source of H5N1 type AIV with recombinant adenovirus expressing granulocyte monocyte colony-stimulating factor (GM-CSF) as an adjuvant. A total of 150-day-old commercial chicks, with AIV-maternal-derived antibody, were divided into 6 groups. The primary vaccination was performed at day 14 followed by a subsequent boosting and intramuscular challenge on day 28 and 42, respectively. Recombinant GM-CSF (rGM-CSF) expressed by adenovirus, named as rAd-GM-CSF, raised the hemagglutination inhibition (HI) titers (log2) against AIV from 7.0 (vaccinate with inactivated vaccine alone) to 8.4 after booster immunization. Moreover, the rGM-CSF addition markedly increased the expression of interferon-γ, interleukin-4, and major histocompatibility complex-II in the lungs, compared with those immunized with inactivated vaccine alone on day 29, that is, 18 h post booster immunization. Following challenge, chicks inoculated with the inactivated AIV vaccine and rAd-GM-CSF together exhibited mild clinical signs and 62% survivals compared to 33% in the group immunized with inactivated AIV vaccine alone. Higher level of HI titers, immune related molecule expressions, and protection ratio demonstrates a good potential of rGM-CSF in improving humoral and cell mediated immune responses of inactivated AIV vaccines.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Adenoviridae / Factor Estimulante de Colonias de Granulocitos y Macrófagos / Gripe Humana / Subtipo H5N1 del Virus de la Influenza A / Vectores Genéticos Límite: Animals / Humans Idioma: En Revista: J Interferon Cytokine Res Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Adenoviridae / Factor Estimulante de Colonias de Granulocitos y Macrófagos / Gripe Humana / Subtipo H5N1 del Virus de la Influenza A / Vectores Genéticos Límite: Animals / Humans Idioma: En Revista: J Interferon Cytokine Res Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos