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Spatial detection of fetal marker genes expressed at low level in adult human heart tissue.
Asp, Michaela; Salmén, Fredrik; Ståhl, Patrik L; Vickovic, Sanja; Felldin, Ulrika; Löfling, Marie; Fernandez Navarro, José; Maaskola, Jonas; Eriksson, Maria J; Persson, Bengt; Corbascio, Matthias; Persson, Hans; Linde, Cecilia; Lundeberg, Joakim.
Afiliación
  • Asp M; Division of Gene Technology, KTH Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.
  • Salmén F; Division of Gene Technology, KTH Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.
  • Ståhl PL; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
  • Vickovic S; Division of Gene Technology, KTH Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.
  • Felldin U; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
  • Löfling M; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
  • Fernandez Navarro J; Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
  • Maaskola J; Division of Gene Technology, KTH Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.
  • Eriksson MJ; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
  • Persson B; Department of Clinical Physiology, Karolinska University Hospital, Stockholm, Sweden.
  • Corbascio M; Department of Molecular Biology, Uppsala University, Science for Life Laboratory, Uppsala, Sweden.
  • Persson H; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Science for Life Laboratory, Stockholm, Sweden.
  • Linde C; Department of Cardiothoracic Surgery and Anesthesiology, Karolinska University Hospital, Solna, Sweden.
  • Lundeberg J; Department of Cardiology, Danderyd Hospital, Stockholm, Sweden.
Sci Rep ; 7(1): 12941, 2017 10 11.
Article en En | MEDLINE | ID: mdl-29021611
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In many studies on heart failure, cardiac biopsies have been analyzed as uniform pieces of tissue with bulk techniques, but this homogenization approach can mask medically relevant phenotypes occurring only in isolated parts of the tissue. This study examines such spatial variations within and between regions of cardiac biopsies. In contrast to standard RNA sequencing, this approach provides a spatially resolved transcriptome- and tissue-wide perspective of the adult human heart, and enables detection of fetal marker genes expressed by minor subpopulations of cells within the tissue. Analysis of patients with heart failure, with preserved ejection fraction, demonstrated spatially divergent expression of fetal genes in cardiac biopsies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores / Regulación del Desarrollo de la Expresión Génica / Feto / Miocardio Tipo de estudio: Diagnostic_studies Aspecto: Patient_preference Límite: Adult / Aged / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores / Regulación del Desarrollo de la Expresión Génica / Feto / Miocardio Tipo de estudio: Diagnostic_studies Aspecto: Patient_preference Límite: Adult / Aged / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido