Sex chromatin analysis of lymphocytes invading host organs in transfusion-associated graft-versus-host disease.
Virchows Arch B Cell Pathol Incl Mol Pathol
; 55(4): 237-9, 1988.
Article
en En
| MEDLINE
| ID: mdl-2900576
We report a method of sex chromatin analysis of lymphocytes separated from host organs in transfusion-associated graft-versus-host disease (GVHD), which enabled the demonstration of invasion by donor lymphocytes. The lymphocytes examined were separated from deparaffinized tissue blocks of skin, spleen and bone marrow from two female patients with transfusion-associated GVHD by incubation in 0.5% pepsin. The tissues had been removed at autopsy and fixed in formalin. Sex chromatin analysis was performed by fluorescence microscopy on separated lymphocytes stained with 0.005% quinacrine dihydrochloride. By this means Y chromatin-positive (i.e. male) lymphocytes were demonstrated in the skin, spleen and bone marrow of both female patients.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatina Sexual
/
Linfocitos
/
Reacción a la Transfusión
/
Enfermedad Injerto contra Huésped
Tipo de estudio:
Etiology_studies
/
Risk_factors_studies
Límite:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Virchows Arch B Cell Pathol Incl Mol Pathol
Año:
1988
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Alemania