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Primers for Castilleja and their utility across Orobanchaceae: I. Chloroplast primers.
Latvis, Maribeth; Mortimer, Sebastian M E; Morales-Briones, Diego F; Torpey, Samuel; Uribe-Convers, Simon; Jacobs, Sarah J; Mathews, Sarah; Tank, David C.
Afiliación
  • Latvis M; Department of Natural Resource Management, South Dakota State University, 1390 College Avenue, Brookings, South Dakota 57007 USA.
  • Mortimer SME; Department of Biological Sciences, University of Idaho, 875 Perimeter Drive, MS 3051, Moscow, Idaho 83844-3051 USA.
  • Morales-Briones DF; Stillinger Herbarium, University of Idaho, 875 Perimeter Drive, MS 1133, Moscow, Idaho 83844-1133 USA.
  • Torpey S; Department of Biological Sciences, University of Idaho, 875 Perimeter Drive, MS 3051, Moscow, Idaho 83844-3051 USA.
  • Uribe-Convers S; Stillinger Herbarium, University of Idaho, 875 Perimeter Drive, MS 1133, Moscow, Idaho 83844-1133 USA.
  • Jacobs SJ; Institute for Bioinformatics and Evolutionary Studies (IBEST), University of Idaho, 875 Perimeter Drive, MS 3051, Moscow, Idaho 83844-3051 USA.
  • Mathews S; Department of Biological Sciences, University of Idaho, 875 Perimeter Drive, MS 3051, Moscow, Idaho 83844-3051 USA.
  • Tank DC; Department of Ecology and Evolutionary Biology, University of Michigan, 830 North University Avenue, Ann Arbor, Michigan 48109 USA.
Appl Plant Sci ; 5(9)2017 Sep.
Article en En | MEDLINE | ID: mdl-28989821
PREMISE OF THE STUDY: Chloroplast primers were developed from genomic data for the taxonomically challenging genus Castilleja. We further tested the broader utility of these primers across Orobanchaceae, identifying a core set of chloroplast primers amplifying across the clade. METHODS AND RESULTS: Using a combination of three low-coverage Castilleja genomes and sequence data from 12 Castilleja plastomes, 76 primer combinations were specifically designed and tested for Castilleja. The primers targeted the most variable portions of the plastome and were validated for their applicability across the clade. Of these, 38 primer combinations were subsequently evaluated in silico and then validated across other major clades in Orobanchaceae. CONCLUSIONS: These results demonstrate the utility of these primers, not only across Castilleja, but for other clades in Orobanchaceae-particularly hemiparasitic lineages-and will contribute to future phylogenetic studies of this important clade of parasitic plants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Appl Plant Sci Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Appl Plant Sci Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos