Dynamic multicolor protein labeling in living cells.

Li, Chenge; Plamont, Marie-Aude; Sladitschek, Hanna L; Rodrigues, Vanessa; Aujard, Isabelle; Neveu, Pierre; Le Saux, Thomas; Jullien, Ludovic; Gautier, Arnaud

Li, Chenge; Plamont, Marie-Aude; Sladitschek, Hanna L; Rodrigues, Vanessa; Aujard, Isabelle; Neveu, Pierre; Le Saux, Thomas; Jullien, Ludovic; Gautier, Arnaud.

Afiliación

Li C; École Normale Supérieure , PSL Research University , UPMC Univ Paris 06 , CNRS , Département de Chimie , PASTEUR , 24 rue Lhomond , 75005 Paris , France.
Plamont MA; Sorbonne Universités , UPMC Univ Paris 06 , ENS , CNRS , PASTEUR , 75005 Paris , France . Email: ludovic.jullien@ens.fr ; Email: arnaud.gautier@ens.fr.
Sladitschek HL; École Normale Supérieure , PSL Research University , UPMC Univ Paris 06 , CNRS , Département de Chimie , PASTEUR , 24 rue Lhomond , 75005 Paris , France.
Rodrigues V; Sorbonne Universités , UPMC Univ Paris 06 , ENS , CNRS , PASTEUR , 75005 Paris , France . Email: ludovic.jullien@ens.fr ; Email: arnaud.gautier@ens.fr.
Aujard I; Cell Biology and Biophysics Unit , European Molecular Biology Laboratory , Meyerhofstr. 1 , D-69117 Heidelberg , Germany.
Neveu P; École Normale Supérieure , PSL Research University , UPMC Univ Paris 06 , CNRS , Département de Chimie , PASTEUR , 24 rue Lhomond , 75005 Paris , France.
Le Saux T; Sorbonne Universités , UPMC Univ Paris 06 , ENS , CNRS , PASTEUR , 75005 Paris , France . Email: ludovic.jullien@ens.fr ; Email: arnaud.gautier@ens.fr.
Jullien L; École Normale Supérieure , PSL Research University , UPMC Univ Paris 06 , CNRS , Département de Chimie , PASTEUR , 24 rue Lhomond , 75005 Paris , France.
Gautier A; Sorbonne Universités , UPMC Univ Paris 06 , ENS , CNRS , PASTEUR , 75005 Paris , France . Email: ludovic.jullien@ens.fr ; Email: arnaud.gautier@ens.fr.

Chem Sci ; 8(8): 5598-5605, 2017 Aug 01.

Article en En | MEDLINE | ID: mdl-28970939

RESUMEN

Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST, hereafter called FAST) is a 14 kDa protein tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of FAST from green-yellow to orange and red. Beyond allowing the multicolor imaging of FAST-tagged proteins in live cells, these fluorogens enable dynamic color switching because of FAST's reversible labeling. This unprecedented behavior allows for selective detection of FAST-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging.

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2017 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido

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