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Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco.
Echchakery, Mohamed; Chicharro, Carmen; Boussaa, Samia; Nieto, Javier; Carrillo, Eugenia; Sheila, Ortega; Moreno, Javier; Boumezzough, Ali.
Afiliación
  • Echchakery M; Ecology and the Environment Laboratory L2E, (URAC 32, CNRST ERACNERS 06), Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco. mohamedechchakery@gmail.com.
  • Chicharro C; WHO Collaborating Centre for Leishmaniasis, Parasitology Service, National Center of Microbiology Institute of Health Carlos III, Majadahonda, Madrid, Spain.
  • Boussaa S; Ecology and the Environment Laboratory L2E, (URAC 32, CNRST ERACNERS 06), Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco.
  • Nieto J; ISPITS-Higher Institute of Nursing and Technical Health Occupations, Ministry of Health, Marrakesh, Morocco.
  • Carrillo E; WHO Collaborating Centre for Leishmaniasis, Parasitology Service, National Center of Microbiology Institute of Health Carlos III, Majadahonda, Madrid, Spain.
  • Sheila O; WHO Collaborating Centre for Leishmaniasis, Parasitology Service, National Center of Microbiology Institute of Health Carlos III, Majadahonda, Madrid, Spain.
  • Moreno J; WHO Collaborating Centre for Leishmaniasis, Parasitology Service, National Center of Microbiology Institute of Health Carlos III, Majadahonda, Madrid, Spain.
  • Boumezzough A; WHO Collaborating Centre for Leishmaniasis, Parasitology Service, National Center of Microbiology Institute of Health Carlos III, Majadahonda, Madrid, Spain.
Parasit Vectors ; 10(1): 454, 2017 Oct 02.
Article en En | MEDLINE | ID: mdl-28969710
BACKGROUND: Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasites' transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. METHODS: Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. RESULTS: A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11 M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). CONCLUSIONS: To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmania tropica / Reservorios de Enfermedades / Leishmaniasis Cutánea / Leishmania infantum / Leishmaniasis Visceral Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Female / Humans / Male País/Región como asunto: Africa Idioma: En Revista: Parasit Vectors Año: 2017 Tipo del documento: Article País de afiliación: Marruecos Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmania tropica / Reservorios de Enfermedades / Leishmaniasis Cutánea / Leishmania infantum / Leishmaniasis Visceral Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Female / Humans / Male País/Región como asunto: Africa Idioma: En Revista: Parasit Vectors Año: 2017 Tipo del documento: Article País de afiliación: Marruecos Pais de publicación: Reino Unido