Screening for concanavalin A binders from a mannose-modified α-helix peptide phage library.
Mol Biosyst
; 13(11): 2222-2225, 2017 Oct 24.
Article
en En
| MEDLINE
| ID: mdl-28967020
Mannose-modified lectin-binding peptides were obtained from an α-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies described in this study will enhance the selection of saccharide-modified ligands through the synergistic effects of sugar and peptide units, with better specificity and affinity towards lectin proteins.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Proteínas Portadoras
/
Concanavalina A
/
Biblioteca de Péptidos
/
Mapeo de Interacción de Proteínas
/
Técnicas de Visualización de Superficie Celular
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Mol Biosyst
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido