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Didox (3,4-dihydroxybenzohydroxamic acid) suppresses IgE-mediated mast cell activation through attenuation of NFκB and AP-1 transcription.
McLeod, Jamie Josephine Avila; Caslin, Heather L; Spence, Andrew J; Kolawole, Elizabeth M; Qayum, Amina Abdul; Paranjape, Anuya; Taruselli, Marcela; Haque, Tamara T; Kiwanuka, Kasalina N; Elford, Howard L; Ryan, John J.
Afiliación
  • McLeod JJA; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Caslin HL; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Spence AJ; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Kolawole EM; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Qayum AA; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Paranjape A; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Taruselli M; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Haque TT; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Kiwanuka KN; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States.
  • Elford HL; Molecules for Health, Inc, Richmond, VA 23219, United States.
  • Ryan JJ; Department of Biology, Virginia Commonwealth University, Richmond, VA, 23284, United States. Electronic address: jjryan@vcu.edu.
Cell Immunol ; 322: 41-48, 2017 Dec.
Article en En | MEDLINE | ID: mdl-28964543
Mast cell activation via the high-affinity IgE receptor (FcεRI) elicits production of inflammatory mediators central to allergic disease. As a synthetic antioxidant and a potent ribonucleotide reductase (RNR) inhibitor, Didox (3,4-dihyroxybenzohydroxamic acid) has been tested in clinical trials for cancer and is an attractive therapeutic for inflammatory disease. We found that Didox treatment of mouse bone marrow-derived mast cells (BMMC) reduced IgE-stimulated degranulation and cytokine production, including IL-6, IL-13, TNF and MIP-1a (CCL3). These effects were consistent using BMMC of different genetic backgrounds and peritoneal mast cells. While the RNR inhibitor hydroxyurea had little or no effect on IgE-mediated function, high concentrations of the antioxidant N-acetylcysteine mimicked Didox-mediated suppression. Furthermore, Didox increased expression of the antioxidant genes superoxide dismutase and catalase, and suppressed DCFH-DA fluorescence, indicating reduced reactive oxygen species production. Didox effects were not due to changes in FcεRI expression or cell viability, suggesting it inhibits signaling required for inflammatory cytokine production. In support of this, we found that Didox reduced FcεRI-mediated AP-1 and NFκB transcriptional activity. Finally, Didox suppressed mast cell-dependent, IgE-mediated passive systemic anaphylaxis in vivo. These data demonstrate the potential use for Didox asa means of antagonizing mast cell responses in allergic disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina E / FN-kappa B / Factor de Transcripción AP-1 / Ácidos Hidroxámicos / Hipersensibilidad / Mastocitos / Antiinflamatorios / Antioxidantes Límite: Animals Idioma: En Revista: Cell Immunol Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina E / FN-kappa B / Factor de Transcripción AP-1 / Ácidos Hidroxámicos / Hipersensibilidad / Mastocitos / Antiinflamatorios / Antioxidantes Límite: Animals Idioma: En Revista: Cell Immunol Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos