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Note: Rapid measurement of fluorescence lifetimes using SiPM detection and waveform sampling.
Tsai, H-M; Souris, J S; Kim, H-J; Cheng, S-H; Chen, L; Lo, L-W; Chen, C-T; Kao, C-M.
Afiliación
  • Tsai HM; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
  • Souris JS; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
  • Kim HJ; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
  • Cheng SH; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
  • Chen L; Department of Surgery, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
  • Lo LW; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
  • Chen CT; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
  • Kao CM; Department of Radiology, The University of Chicago, Chicago, Illinois 60637, USA.
Rev Sci Instrum ; 88(9): 096107, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28964223
In fluorescence spectroscopy and imaging, fluorescence lifetime measurement-assessing the average time fluorophores spend in their excited state before returning to their ground state-offers a number of advantages over quantifying fluorescence intensities that include resistance to photo-bleaching and independence from fluorophore concentration, excitation intensity, and measurement methodology. Despite growing interest, fluorescence lifetime techniques frequently mandate relatively complex instrumentation, slow data acquisition rates, and significant data analyses. In this work, we demonstrate the feasibility of measuring fluorescence lifetimes using off-the-shelf analog silicon photomultipliers and switched-capacitor array waveform sampling techniques, with precision matching that of much larger and more elaborate commercial instruments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Rev Sci Instrum Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Rev Sci Instrum Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos