Your browser doesn't support javascript.
loading
Ancient permafrost staphylococci carry antibiotic resistance genes.
Kashuba, Elena; Dmitriev, Alexey A; Kamal, Shady Mansour; Melefors, Ojar; Griva, Gennady; Römling, Ute; Ernberg, Ingemar; Kashuba, Vladimir; Brouchkov, Anatoli.
Afiliación
  • Kashuba E; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
  • Dmitriev AA; Laboratory of Cancer Genetics, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine.
  • Kamal SM; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
  • Melefors O; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
  • Griva G; Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University in Egypt, New Cairo, Egypt.
  • Römling U; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
  • Ernberg I; Siberian Branch of RAS, Institute of the Earth Cryosphere, Tyumen, Russia.
  • Kashuba V; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
  • Brouchkov A; Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden.
Microb Ecol Health Dis ; 28(1): 1345574, 2017.
Article en En | MEDLINE | ID: mdl-28959177
Background: Permafrost preserves a variety of viable ancient microorganisms. Some of them can be cultivated after being kept at subzero temperatures for thousands or even millions of years. Objective: To cultivate bacterial strains from permafrost. Design: We isolated and cultivated two bacterial strains from permafrost that was obtained at Mammoth Mountain in Siberia and attributed to the Middle Miocene. Bacterial genomic DNA was sequenced with 40-60× coverage and high-quality contigs were assembled. The first strain was assigned to Staphylococcus warneri species (designated MMP1) and the second one to Staphylococcus hominis species (designated MMP2), based on the classification of 16S ribosomal RNA genes and genomic sequences. Results: Genomic sequence analysis revealed the close relation of the isolated ancient bacteria to the modern bacteria of this species. Moreover, several genes associated with resistance to different groups of antibiotics were found in the S. hominis MMP2 genome. Conclusions: These findings supports a hypothesis that antibiotic resistance has an ancient origin. The enrichment of cultivated bacterial communities with ancient permafrost strains is essential for the analysis of bacterial evolution and antibiotic resistance.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microb Ecol Health Dis Año: 2017 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Suecia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microb Ecol Health Dis Año: 2017 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Suecia