In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics.
Mem Inst Oswaldo Cruz
; 112(10): 655-663, 2017 Oct.
Article
en En
| MEDLINE
| ID: mdl-28953993
BACKGROUND: The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be considered for immunodiagnostic purposes. OBJECTIVES: To analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development. METHODS: We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in the GenBank database. We also evaluated their similarity with homologous proteins from other viruses. FINDINGS: The mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein would most likely produce false negative results due to its high divergence. MAIN CONCLUSIONS: We have identified specific regions in each of these three proteins that have higher potential to produce specific results, and their combined utilisation should be considered for immunoassay development.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Variación Genética
/
Proteínas Virales
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Virus Sincitial Respiratorio Humano
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Infecciones por Virus Sincitial Respiratorio
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil