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Methanobrevibacter attenuation via probiotic intervention reduces flatulence in adult human: A non-randomised paired-design clinical trial of efficacy.
Seo, Minseok; Heo, Jaeyoung; Yoon, Joon; Kim, Se-Young; Kang, Yoon-Mo; Yu, Jihyun; Cho, Seoae; Kim, Heebal.
Afiliación
  • Seo M; C&K Genomics, Seoul National University Research Park, Seoul, Republic of Korea.
  • Heo J; Department of Animal Biotechnology, Chonbuk National University, Jeonju, Republic of Korea.
  • Yoon J; Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.
  • Kim SY; R&D Center, CTCBIO, Inc., Hwaseong-si, Gyeonggi-do, Republic of Korea.
  • Kang YM; R&D Center, CTCBIO, Inc., Hwaseong-si, Gyeonggi-do, Republic of Korea.
  • Yu J; Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
  • Cho S; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
  • Kim H; C&K Genomics, Seoul National University Research Park, Seoul, Republic of Korea.
PLoS One ; 12(9): e0184547, 2017.
Article en En | MEDLINE | ID: mdl-28937980
TRIAL DESIGN: The aim of this study was to investigate which of the gut microbes respond to probiotic intervention, as well as study whether they are associated with gastrointestinal symptoms in a healthy adult human. For the experimental purpose, twenty-one healthy adults were recruited and received probiotic mixture, which is composed of five Lactobacilli strains and two Bifidobacteria strains, once a day for 60 days. Defecation survey and Bioelectrical Impedance Analysis were conducted pre- and post-administration to measure phenotypic differences. Stool samples of the subjects were collected twice. METHODS: The statistical analysis was performed for pair designed metagenome data with 11 phenotypic records of the bioelectrical impedance body composition analyzer and 6 responses of the questionnaires about gastrointestinal symptom. Furthemore, correlation-based network analysis was conducted for exploring complex relationships among microbiome communities. RESULTS: The abundances of Citrobacter, Klebsiella, and Methanobrevibacter were significantly reduced, which are strong candidates to be highly affected by the probiotic administration. In addition, interaction effects were observed between flatulence symptom attenuation and decreasing patterns of the Methanobrevibacter abundance. CONCLUSIONS: These results reveal that probiotic intervention modulated the composition of gut microbiota and reduced the abundance of potential pathogens (i.e. Citrobacter and Klebsiella). In addition, methanogens (i.e. Methanobrevibacter) associated with the gastrointestinal symptom in an adult human.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Probióticos / Methanobrevibacter / Flatulencia / Microbioma Gastrointestinal Tipo de estudio: Clinical_trials Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Probióticos / Methanobrevibacter / Flatulencia / Microbioma Gastrointestinal Tipo de estudio: Clinical_trials Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos