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Novel H-shunt Venovenous Bypass for Liver Transplantation in Cynomolgus Macaques.
Kato, Yojiro; Griesemer, Adam D; Wu, Anette; Sondermeijer, Hugo P; Weiner, Joshua I; Duran-Struuck, Raimon; Martinez, Mercedes; Slate, Andrea R; Romanov, Alexander; Lefkowitch, Jay H; Sykes, Megan; Kato, Tomoaki.
Afiliación
  • Kato Y; Departments of Surgery, Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Griesemer AD; Departments of Surgery, Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Wu A; Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Sondermeijer HP; Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Weiner JI; Departments of Surgery, Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Duran-Struuck R; Departments of Surgery, Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Martinez M; Departments of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Slate AR; Institute of Comparative Medicine, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Romanov A; Institute of Comparative Medicine, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Lefkowitch JH; Departments of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Sykes M; Columbia Center for Translational Immunology, College of Physicians and Surgeons, Columbia University, New York, New York.
  • Kato T; Departments of Surgery, College of Physicians and Surgeons, Columbia University, New York, New York;, Email: tk2388@cumc.columbia.edu.
Comp Med ; 67(5): 436-441, 2017 Oct 01.
Article en En | MEDLINE | ID: mdl-28935006
Cynomolgus monkeys are often used in preclinical transplantation research. Performing liver transplantation in cynomolgus monkeys is challenging because they poorly tolerate portal vein clamping during the anhepatic phase. Finding an alternative to portal vein clamping is necessary before preclinical liver transplant models can be performed with reliable outcomes. We used 3 different techniques to perform 5 liver transplants in male cynomolgus macaques (weight, 7.4-10.8 kg; mismatched for MHC I and II; matched for ABO). In procedure A, we clamped the portal vein briefly, as in human transplants, as well as the superior mesentery artery to minimize congestion at the expense of temporary ischemia (n = 2). In procedure B, we performed a temporary portocaval shunt with extracorporeal venovenous bypass (n = 1). For procedure C, we developed an H-shunt system (modified portocaval shunt) with extracorporeal bypass (n = 2). Postoperative immunosuppression comprised cyclosporine A, mycophenolate mofetil, and steroids. Recipients in procedure A developed hemodynamic instability and were euthanized within 2 d. The recipient that underwent procedure B was euthanized within 11 d due to inferior vena caval thrombosis. The H-shunt in procedure C led to minimal PV congestion during the anhepatic phase, and both recipients reached the 21-d survival endpoint with good graft function. Our novel H-shunt bypass system resulted in successful liver transplantation in cynomolgus macaques, with long-term posttransplant survival possible. This technical innovation makes possible the use of cynomolgus monkeys for preclinical liver transplant tolerance models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Derivación Portocava Quirúrgica / Trasplante de Hígado / Ciclosporina / Inmunosupresores / Macaca fascicularis Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: Comp Med Asunto de la revista: MEDICINA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Derivación Portocava Quirúrgica / Trasplante de Hígado / Ciclosporina / Inmunosupresores / Macaca fascicularis Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans / Male Idioma: En Revista: Comp Med Asunto de la revista: MEDICINA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos