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Baicalin alleviates radiation-induced epithelial-mesenchymal transition of primary type II alveolar epithelial cells via TGF-ß and ERK/GSK3ß signaling pathways.
Lu, Jinhua; Zhong, Yazhen; Lin, Zechen; Lin, Xianlei; Chen, Zhaohui; Wu, Xuping; Wang, Nan; Zhang, Haiqiao; Huang, Siyu; Zhu, Yuan; Wang, Yuanyuan; Lin, Shengyou.
Afiliación
  • Lu J; Hangzhou Cancer Hospital, Hangzhou Hospital affiliated to Nanjing Medical University, Hangzhou Cancer Center, Hangzhou, Zhejiang, 310000, PR China.
  • Zhong Y; Hangzhou Cancer Hospital, Hangzhou Hospital affiliated to Nanjing Medical University, Hangzhou Cancer Center, Hangzhou, Zhejiang, 310000, PR China.
  • Lin Z; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Lin X; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Chen Z; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Wu X; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Wang N; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Zhang H; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Huang S; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Zhu Y; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Wang Y; Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, 310000, PR China.
  • Lin S; Hangzhou Cancer Hospital, Hangzhou Hospital affiliated to Nanjing Medical University, Hangzhou Cancer Center, Hangzhou, Zhejiang, 310000, PR China. Electronic address: linsy0628@163.com.
Biomed Pharmacother ; 95: 1219-1224, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28931214
BACKGROUND: Radiation therapy is commonly used to treat thoracic malignancies. However, it may lead to severe lung pneumonitis and ultimately fibrosis. Irradiation has been reported to increase epithelial-mesenchymal transition (EMT) of type II alveolar epithelial cells (AEC), which play an important role in pulmonary fibrosis. The transforming growth factor-ß (TGF-ß) and ERK/glycogen synthase kinase 3ß (GSK3ß) pathways are critically involved in radiation-induced EMT. In the present study, we investigated whether baicalin was a novel therapeutic candidate for radiation-induced EMT in type II AEC. METHODS: Primary type II AEC were isolated and treated with 60Co γ-rays and a series doses of baicalin (2µM, 10µM and 50µM). The ultrastructure and morphology changes were observed by transmission electron microscopy and optical microscopy, respectively. Protein expression was determined by western blotting analysis. Immunofluorescence staining was performed to detect the nuclear translocation of Snail. RESULTS: After irradiation, type II AEC displayed a mesenchymal-like morphology accompanied by a decrease in E-cadherin expression, an increase in the expression of Vimentin and α-SMA. Nuclear translocation of Snail, the activation of TGF-ß/Smad pathway, and the inactivation of GSK3ß were prominent in radiation-treated cells. Baicalin significantly attenuated the effects of radiation on type II AEC. CONCLUSIONS: Baicalin may a useful radioprotective agent through suppressing the EMT of type II AEC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Flavonoides / Factor de Crecimiento Transformador beta / Sistema de Señalización de MAP Quinasas / Células Epiteliales Alveolares / Transición Epitelial-Mesenquimal / Glucógeno Sintasa Quinasa 3 beta Límite: Animals Idioma: En Revista: Biomed Pharmacother Año: 2017 Tipo del documento: Article Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Flavonoides / Factor de Crecimiento Transformador beta / Sistema de Señalización de MAP Quinasas / Células Epiteliales Alveolares / Transición Epitelial-Mesenquimal / Glucógeno Sintasa Quinasa 3 beta Límite: Animals Idioma: En Revista: Biomed Pharmacother Año: 2017 Tipo del documento: Article Pais de publicación: Francia