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Hydrogel Tethering Enhances Interdomain Stabilization of Single-Chain Antibodies.
Xiong, Yijia; Ford, Nicole R; Hecht, Karen A; Roesijadi, Guritno; Squier, Thomas C.
Afiliación
  • Xiong Y; Department of Basic Medical Sciences, Western University of Health Sciences , Lebanon, Oregon 97355, United States.
  • Ford NR; Marine Biotechnology, Pacific Northwest National Laboratory , Sequim, Washington 98382, United States.
  • Hecht KA; Marine Biotechnology, Pacific Northwest National Laboratory , Sequim, Washington 98382, United States.
  • Roesijadi G; Marine Biotechnology, Pacific Northwest National Laboratory , Sequim, Washington 98382, United States.
  • Squier TC; Department of Microbiology, Oregon State University , Corvallis, Oregon 97331, United States.
Bioconjug Chem ; 28(11): 2804-2814, 2017 11 15.
Article en En | MEDLINE | ID: mdl-28930443
Here, we identify the importance of molecular crowding agents in the functional stabilization of scFv antibodies. Antibodies were tethered through an engineered calmodulin (CaM)-binding peptide into a stimulus-responsive hydrogel composed of poly(ethylene glycol) (PEG)-functionalized CaM. Macromolecular crowding is modulated by transient heating, which decreases effective pore sizes. Using a fluorescent ligand bound to the scFv, frequency-domain fluorescence spectroscopy was used to assess the structural coupling between the VH and the VL domains and relationships with functional stabilization. There is minimal structural coupling between the VH and the VL domains in solution, as is apparent from the substantial rotational mobility for the bound ligand, that is suggestive of an independent mobility for the VH and the VL domains. In comparison, the hydrogel matrix acts to structurally couple the VH and the VL domains, resulting in a reduction in rotational mobility and a retention of ligand binding in the presence of 8.0 M urea. Under these same conditions, ligand binding is disrupted for scFv antibodies in solution. Increases in the stabilization of scFv antibodies in hydrogels is not simply the result of molecular crowding because decreases in pore size act to destabilize ligand binding. Rather, our results suggest that the functional stabilization of the scFv antibody within the PEG hydrogel matrix includes important factors involving protein solvation that stabilize interdomain interactions between the VH and the VL domains necessary for ligand binding.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Polietilenglicoles / Hidrogel de Polietilenoglicol-Dimetacrilato / Anticuerpos Inmovilizados / Anticuerpos de Cadena Única Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Bioconjug Chem Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Polietilenglicoles / Hidrogel de Polietilenoglicol-Dimetacrilato / Anticuerpos Inmovilizados / Anticuerpos de Cadena Única Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Bioconjug Chem Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos