Your browser doesn't support javascript.
loading
A Genetic Screen for Impaired Systemic RNAi Highlights the Crucial Role of DICER-LIKE 2.
Taochy, Christelle; Gursanscky, Nial R; Cao, Jiangling; Fletcher, Stephen J; Dressel, Uwe; Mitter, Neena; Tucker, Matthew R; Koltunow, Anna M G; Bowman, John L; Vaucheret, Hervé; Carroll, Bernard J.
Afiliación
  • Taochy C; School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Gursanscky NR; Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique AgroParisTech Centre National de la Recherche Scientifique, Université Paris-Saclay, 78000 Versailles, France.
  • Cao J; School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Fletcher SJ; School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Dressel U; School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Mitter N; Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Tucker MR; School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Koltunow AMG; School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Bowman JL; Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Queensland 4072, Australia.
  • Vaucheret H; Commonwealth Scientific and Industrial Research Organization Agriculture, Waite Campus, Urrbrae, South Australia 5064, Australia.
  • Carroll BJ; School of Agriculture, Food, and Wine, University of Adelaide, Waite Campus, Adelaide, South Australia 5064, Australia.
Plant Physiol ; 175(3): 1424-1437, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28928141
Posttranscriptional gene silencing (PTGS) of transgenes involves abundant 21-nucleotide small interfering RNAs (siRNAs) and low-abundance 22-nucleotide siRNAs produced from double-stranded RNA (dsRNA) by DCL4 and DCL2, respectively. However, DCL2 facilitates the recruitment of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) to ARGONAUTE 1-derived cleavage products, resulting in more efficient amplification of secondary and transitive dsRNA and siRNAs. Here, we describe a reporter system where RDR6-dependent PTGS is initiated by restricted expression of an inverted-repeat dsRNA specifically in the Arabidopsis (Arabidopsis thaliana) root tip, allowing a genetic screen to identify mutants impaired in RDR6-dependent systemic PTGS. Our screen identified dcl2 but not dcl4 mutants. Moreover, grafting experiments showed that DCL2, but not DCL4, is required in both the source rootstock and the recipient shoot tissue for efficient RDR6-dependent systemic PTGS. Furthermore, dcl4 rootstocks produced more DCL2-dependent 22-nucleotide siRNAs than the wild type and showed enhanced systemic movement of PTGS to grafted shoots. Thus, along with its role in recruiting RDR6 for further amplification of PTGS, DCL2 is crucial for RDR6-dependent systemic PTGS.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Genéticas / Arabidopsis / Proteínas de Ciclo Celular / Proteínas de Arabidopsis / Interferencia de ARN / Ribonucleasa III Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Genéticas / Arabidopsis / Proteínas de Ciclo Celular / Proteínas de Arabidopsis / Interferencia de ARN / Ribonucleasa III Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2017 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos