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Comprehensive proteomics analysis of exosomes derived from human seminal plasma.
Yang, C; Guo, W-B; Zhang, W-S; Bian, J; Yang, J-K; Zhou, Q-Z; Chen, M-K; Peng, W; Qi, T; Wang, C-Y; Liu, C-D.
Afiliación
  • Yang C; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Guo WB; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Zhang WS; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Bian J; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Yang JK; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Zhou QZ; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Chen MK; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Peng W; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
  • Qi T; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
  • Wang CY; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
  • Liu CD; Department of Urology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
Andrology ; 5(5): 1007-1015, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28914500
Exosomes are membranous nanovesicles of endocytic origin that carry and transfer regulatory bioactive molecules and mediate intercellular communication between cells and tissues. Although seminal exosomes have been identified in human seminal plasma, their exact composition and possible physiologic function remain unknown. The objective of this study was to perform a comprehensive proteomics analysis of exosomes derived from human seminal plasma. Seminal exosomes were isolated and purified from 12 healthy donors using a 30% sucrose cushion-based exosome-isolation protocol, followed by characterization by western blot, transmission electron microscopy, and nanoparticle tracking analysis before performing extensive liquid chromatography tandem mass spectrometry proteomics analysis. The identified proteins were analyzed by bioinformatics analysis, and seminal exosomes-associated proteins were selectively validated by western blot. A total of 1474 proteins were identified in all seminal exosomes samples, with Gene Ontology analysis demonstrating that these identified seminal exosomes-associated proteins were mostly linked to 'exosomes,' 'cytoplasm,' and 'cytosol.' Bioinformatics analysis indicated that these proteins were mainly involved in biologic processes, including metabolism, energy pathways, protein metabolism, cell growth and maintenance, and transport. Of these identified proteins, PHGDH, LGALS3BP, SEMG1, ACTB, GAPDH, and the exosomal-marker protein ALIX were validated by western blot. This study provided a more comprehensive description of the seminal exosomes proteome and could also be a resource for further screening of biomarkers and comparative proteomics studies, including those associated with male infertility and prostate cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Proteoma Límite: Adult / Humans / Male Idioma: En Revista: Andrology Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Proteoma Límite: Adult / Humans / Male Idioma: En Revista: Andrology Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido