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Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources.
Alderisio, K A; Villegas, L F; Ware, M W; McDonald, L A; Xiao, L; Villegas, E N.
Afiliación
  • Alderisio KA; New York City Department of Environmental Protection, Valhalla and Kingston, NY, USA.
  • Villegas LF; CB&I Federal Services, Inc., Cincinnati, OH, USA.
  • Ware MW; National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH, USA.
  • McDonald LA; New York City Department of Environmental Protection, Valhalla and Kingston, NY, USA.
  • Xiao L; National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Villegas EN; National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH, USA.
J Appl Microbiol ; 123(6): 1607-1613, 2017 Dec.
Article en En | MEDLINE | ID: mdl-28910512
AIM: USEPA Method 1623, or its equivalent, is currently used to monitor for protozoan contamination of surface drinking water sources worldwide. At least three approved staining kits used for detecting Cryptosporidium and Giardia are commercially available. This study focuses on understanding the differences among staining kits used for Method 1623. METHODS AND RESULTS: Merifluor and EasyStain labelling kits were used to monitor Cryptosporidium oocyst and Giardia cyst densities in New York City's raw surface water sources. In the year following a change to the approved staining kits for use with Method 1623, an anomaly was noted in the occurrence of Giardia cysts in New York City's raw surface water. Specifically, Merifluor-stained samples had higher Giardia cyst densities as compared with those stained with EasyStain. Side by side comparison revealed significantly lower fluorescence intensities of Giardia muris as compared with Giardia duodenalis cysts when labelled with EasyStain. CONCLUSIONS: This study showed very poor fluorescence intensity signals by EasyStain on G. muris cysts resulting in lower cyst counts, while Merifluor, with its broader Giardia cyst staining specificity, resulted in higher cyst counts, when using Methods 1623. SIGNIFICANCE AND IMPACT OF THE STUDY: These results suggest that detected Giardia cyst concentrations are dependent on the staining kits used, which can result in a more or less conservative estimation of occurrences and densities of zoonotic Giardia cysts by detecting a broader range of Giardia species/Assemblages.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua Potable / Calidad del Agua / Cryptosporidium / Giardia Tipo de estudio: Evaluation_studies País/Región como asunto: America do norte Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua Potable / Calidad del Agua / Cryptosporidium / Giardia Tipo de estudio: Evaluation_studies País/Región como asunto: America do norte Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido