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ZNF143 protein is an important regulator of the myeloid transcription factor C/EBPα.
Gonzalez, David; Luyten, Annouck; Bartholdy, Boris; Zhou, Qiling; Kardosova, Miroslava; Ebralidze, Alex; Swanson, Kenneth D; Radomska, Hanna S; Zhang, Pu; Kobayashi, Susumu S; Welner, Robert S; Levantini, Elena; Steidl, Ulrich; Chong, Gilbert; Collombet, Samuel; Choi, Min Hee; Friedman, Alan D; Scott, Linda M; Alberich-Jorda, Meritxell; Tenen, Daniel G.
Afiliación
  • Gonzalez D; From the Cancer Science Institute, National University of Singapore, 117599 Singapore.
  • Luyten A; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Bartholdy B; From the Cancer Science Institute, National University of Singapore, 117599 Singapore.
  • Zhou Q; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Kardosova M; From the Cancer Science Institute, National University of Singapore, 117599 Singapore.
  • Ebralidze A; the Institute of Molecular Genetics of the ASCR, Prague 142 20, Czech Republic.
  • Swanson KD; the Childhood Leukaemia Investigation Prague, Second Faculty of Medicine Charles University, University Hospital Motol, Prague 150 06, Czech Republic.
  • Radomska HS; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Zhang P; the Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115.
  • Kobayashi SS; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Welner RS; The Ohio State University, Comprehensive Cancer Center, Columbus, Ohio 43210, and.
  • Levantini E; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Steidl U; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Chong G; the Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115.
  • Collombet S; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Choi MH; the Hematology/Oncology Department, University of Alabama at Birmingham, Birmingham, Alabama 35294.
  • Friedman AD; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Scott LM; the Institute of Biomedical Technologies, National Research Council, 56124 Pisa, Italy.
  • Alberich-Jorda M; the Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115.
  • Tenen DG; the Department of Cell Biology, and Department of Medicine (Oncology), Albert Einstein College of Medicine, New York, New York 10461.
J Biol Chem ; 292(46): 18924-18936, 2017 11 17.
Article en En | MEDLINE | ID: mdl-28900037
The transcription factor C/EBPα is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, ∼100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBPα expression. This sequence is present in the genes encoding C/EBPα in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBPα expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBPα in myeloid cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación Transcripcional / Transactivadores / Regiones Promotoras Genéticas / Células Mieloides / Proteína alfa Potenciadora de Unión a CCAAT Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Biol Chem Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación Transcripcional / Transactivadores / Regiones Promotoras Genéticas / Células Mieloides / Proteína alfa Potenciadora de Unión a CCAAT Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Biol Chem Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos