Influence of genetic background of engineered xylose-fermenting industrial Saccharomyces cerevisiae strains for ethanol production from lignocellulosic hydrolysates.
J Ind Microbiol Biotechnol
; 44(11): 1575-1588, 2017 Nov.
Article
en En
| MEDLINE
| ID: mdl-28891041
An industrial ethanol-producing Saccharomyces cerevisiae strain with genes of fungal oxido-reductive pathway needed for xylose fermentation integrated into its genome (YRH1415) was used to obtain haploids and diploid isogenic strains. The isogenic strains were more effective in metabolizing xylose than YRH1415 strain and able to co-ferment glucose and xylose in the presence of high concentrations of inhibitors resulting from the hydrolysis of lignocellulosic biomass (switchgrass). The rate of xylose consumption did not appear to be affected by the ploidy of strains or the presence of two copies of the xylose fermentation genes but by heterozygosity of alleles for xylose metabolism in YRH1415. Furthermore, inhibitor tolerance was influenced by the heterozygous genome of the industrial strain, which also showed a marked influenced on tolerance to increasing concentrations of toxic compounds, such as furfural. In this work, selection of haploid derivatives was found to be a useful strategy to develop efficient xylose-fermenting industrial yeast strains.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Xilosa
/
Regulación Fúngica de la Expresión Génica
/
Proteínas de Saccharomyces cerevisiae
/
Etanol
/
Lignina
Idioma:
En
Revista:
J Ind Microbiol Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
/
MICROBIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Alemania