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Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats.
Dipaz-Berrocal, D J; Sá, N A R; Guerreiro, D D; Celestino, J J H; Leiva-Revilla, J; Alves, B G; Alves, K A; Santos, R R; Cibin, F W S; Rodrigues, A P R; Figueiredo, J R.
Afiliación
  • Dipaz-Berrocal DJ; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Sá NAR; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Guerreiro DD; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Celestino JJH; Institute of Health Sciences, University of International Integration Lusophone African-Brazilian, Acarape, CE, Brazil.
  • Leiva-Revilla J; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Alves BG; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Alves KA; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Santos RR; Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Castanhal, Pará, Brazil.
  • Cibin FWS; Laboratory of Reproduction Biotechnology (Biotech), Campus Uruguaiana, Federal University of Pampa, Uruguaiana, Brazil.
  • Rodrigues APR; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil.
  • Figueiredo JR; Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles - LAMOFOPA, Faculty of Veterinary, University of Ceará, Fortaleza, Brazil. Electronic address: jrf.lamofopapapers@gmail.com.br.
Anim Reprod Sci ; 185: 118-127, 2017 Oct.
Article en En | MEDLINE | ID: mdl-28882328
The aim of the present study was to investigate the influence of two insulin concentrations (10ng/mL and 10µg/mL) combined or in the absence of BMP15 and/or GDF9, on the in vitro survival and development of preantral follicles of goat ovarian tissue. Ovarian slices from the same goat ovary pair were randomly assigned to a non-cultured control treatment or to be in vitro cultured for 1 or 7days in α-MEM containing 10ng/mL (Low) or 10µg/mL (High) of insulin in the absence or presence of BMP15 and/or GDF9. With the low insulin treatment, there was a greater percentage of normal follicles than with the high insulin treatment. The addition of BMP15 alone or in association with GDF9 to the medium containing low insulin resulted in a lesser percentage of normal follicles (P<0.05). The addition of BMP15 and GDF9 separately or in combination with the high insulin concentration enhanced the percentage of normal follicles. On day 7 of culture, the use of medium containing low insulin alone or high insulin supplemented with BMP15 and BMP15+GDF9 resulted in a greater percentage of secondary follicles than the non-cultured control, although follicles cultured with low insulin were smaller than those from the control group and had greater rates of oxidative stress. In conclusion, in the presence of physiological concentrations of insulin (10ng/mL), medium supplementation with GDF9 and BMP15 alone or in combination is unnecessary for normal follicle development in vitro.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cabras / Proteína Morfogenética Ósea 15 / Factor 9 de Diferenciación de Crecimiento / Folículo Ovárico / Insulina Límite: Animals Idioma: En Revista: Anim Reprod Sci Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cabras / Proteína Morfogenética Ósea 15 / Factor 9 de Diferenciación de Crecimiento / Folículo Ovárico / Insulina Límite: Animals Idioma: En Revista: Anim Reprod Sci Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos