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Dose Titration of Walleye Dermal Sarcoma (WDS) Tumor Filtrate.
Getchell, R G; Wooster, G A; Sutton, C A; Casey, J W; Bowser, P R.
Afiliación
  • Getchell RG; a Aquatic Animal Health Program, Department of Microbiology and Immunology , College of Veterinary Medicine, Cornell University , Ithaca , New York , 14853 , USA.
  • Wooster GA; a Aquatic Animal Health Program, Department of Microbiology and Immunology , College of Veterinary Medicine, Cornell University , Ithaca , New York , 14853 , USA.
  • Sutton CA; a Aquatic Animal Health Program, Department of Microbiology and Immunology , College of Veterinary Medicine, Cornell University , Ithaca , New York , 14853 , USA.
  • Casey JW; a Aquatic Animal Health Program, Department of Microbiology and Immunology , College of Veterinary Medicine, Cornell University , Ithaca , New York , 14853 , USA.
  • Bowser PR; a Aquatic Animal Health Program, Department of Microbiology and Immunology , College of Veterinary Medicine, Cornell University , Ithaca , New York , 14853 , USA.
J Aquat Anim Health ; 14(4): 247-253, 2002 Dec.
Article en En | MEDLINE | ID: mdl-28880799
Walleyes Stizostedion vitreum were challenged with a topical application of a dilution series of cell-free dermal sarcoma tumor filtrates to determine the minimum dose of virus needed to induce these walleye tumors. A series of six 10-fold dilutions of the filtrate were applied to the side of the fish, which were allowed to develop grossly visible tumors at 15°C for 20 weeks. Quantification of the virus in the filtrates was accomplished by quantitative (real-time) reverse transcriptase-polymerase chain reaction. We determined that there are approximately 1010 viral RNA copies in 100 µL of walleye dermal sarcoma inoculum. The minimum dose of walleye dermal sarcoma virus that could induce tumors by the topical challenge method was the 1,000-fold dilution of this 1010 inoculum, or approximately 107 viral RNA copies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Aquat Anim Health Asunto de la revista: MEDICINA VETERINARIA Año: 2002 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Aquat Anim Health Asunto de la revista: MEDICINA VETERINARIA Año: 2002 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos