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Effects of soy isoflavones on the concentration of hyaluronic acid in the vagina of type 1 diabetic rats.
F Carbonel, A A; Azevedo Lima, P D; Lim, J J; Teixeira Borges, F; Rodrigues da Silva Sasso, G; Portugal Fuchs, L F; S Simões, R; Chada Baracat, E; Soares, J M; J Simões, M.
Afiliación
  • F Carbonel AA; a Department of Morphology and Genetics , Paulista School of Medicine/Federal University of São Paulo, EPM/UNIFESP , São Paulo , Brazil.
  • Azevedo Lima PD; b Department of Cellular and Molecular Medicine , University of Ottawa , Ottawa , Canada.
  • Lim JJ; b Department of Cellular and Molecular Medicine , University of Ottawa , Ottawa , Canada.
  • Teixeira Borges F; c Department of Nephrology , Paulista School of Medicine/Federal University of São Paulo, EPM/UNIFESP , São Paulo , Brazil.
  • Rodrigues da Silva Sasso G; a Department of Morphology and Genetics , Paulista School of Medicine/Federal University of São Paulo, EPM/UNIFESP , São Paulo , Brazil.
  • Portugal Fuchs LF; d Department of Obstetrics and Gynecology , Medicine Faculty of University of São Paulo, FMUSP , São Paulo , Brazil.
  • S Simões R; d Department of Obstetrics and Gynecology , Medicine Faculty of University of São Paulo, FMUSP , São Paulo , Brazil.
  • Chada Baracat E; d Department of Obstetrics and Gynecology , Medicine Faculty of University of São Paulo, FMUSP , São Paulo , Brazil.
  • Soares JM; d Department of Obstetrics and Gynecology , Medicine Faculty of University of São Paulo, FMUSP , São Paulo , Brazil.
  • J Simões M; a Department of Morphology and Genetics , Paulista School of Medicine/Federal University of São Paulo, EPM/UNIFESP , São Paulo , Brazil.
Climacteric ; 20(6): 564-570, 2017 Dec.
Article en En | MEDLINE | ID: mdl-28866964
OBJECTIVE: To assess the effects of isoflavones and 17ß-estradiol on the vaginal epithelium extracellular matrix and hyaluronic acid (HA) in the diabetic rat model. METHODS: Sixty adult, virgin, female rats underwent ovariectomy, then randomization into six groups of ten animals each: GI, sham ovariectomized control animals; GII, sham ovariectomized control diabetic animals; GIII, control ovariectomized rats receiving propylene glycol vehicle; GIV, control ovariectomized diabetic animals receiving propylene glycol vehicle; GV, diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage); GVI, ovariectomized diabetic rats treated with estrogen (17ß-estradiol, 10 mg/kg, subcutaneously). Treatment took place over 30 consecutive days. After euthanasia, a portion of the vagina was immersed in liquid nitrogen for RT-qPCR and Western blotting. Another portion was processed for paraffin embedding. Sections were stained with hematoxylin & eosin for histomorphometry and Picro Sirius Red for collagen quantification. RESULTS: Vaginal epithelium histomorphometry in GIII (15.3 ± 1.1 µm) and GIV (14.5 ± 1.8 µm) was thinner than in GV (41.3 ± 1.5 µm) and GVI (74.3 ± 1.6 µm). There was an increase in collagen content in GV (84.1 ± 1.2 µm) and GVI (88.2 ± 1.7 µm). HA quantification was higher in GV (0.38 ± 1.1 µg/mg) and GVI (0.49 ± 1.4 µg/mg) when compared with GIII (0.12 ± 1.1 µg/mg) and GIV (0.10 ± 1.2 µg/mg), p < 0.05. CONCLUSIONS: Soy isoflavones increase hyaluronic acid concentration in the vagina of diabetic ovariectomized rats. Such findings might help to attenuate the effects of vulvovaginal atrophy in women.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Vagina / Diabetes Mellitus Experimental / Ácido Hialurónico / Isoflavonas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Climacteric Asunto de la revista: GINECOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Vagina / Diabetes Mellitus Experimental / Ácido Hialurónico / Isoflavonas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Climacteric Asunto de la revista: GINECOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido