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Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus.
Stachyra, Anna; Rak, Monika; Redkiewicz, Patrycja; Madeja, Zbigniew; Gawarecka, Katarzyna; Chojnacki, Tadeusz; Swiezewska, Ewa; Masnyk, Marek; Chmielewski, Marek; Sirko, Agnieszka; Góra-Sochacka, Anna.
Afiliación
  • Stachyra A; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
  • Rak M; Department of Cell Biology, Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Gronostajowa 7, 30-387, Kraków, Poland.
  • Redkiewicz P; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
  • Madeja Z; Department of Cell Biology, Jagiellonian University, Faculty of Biochemistry, Biophysics and Biotechnology, Gronostajowa 7, 30-387, Kraków, Poland.
  • Gawarecka K; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
  • Chojnacki T; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
  • Swiezewska E; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
  • Masnyk M; Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
  • Chmielewski M; Institute of Organic Chemistry Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
  • Sirko A; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland.
  • Góra-Sochacka A; Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. annag@ibb.waw.pl.
Virol J ; 14(1): 168, 2017 09 02.
Article en En | MEDLINE | ID: mdl-28865454
BACKGROUND: Cationic derivatives of polyprenols (trimethylpolyprenylammonium iodides - PTAI) with variable chain length between 6 and 15 isoprene units prepared from naturally occurring poly-cis-prenols were tested as DNA vaccine carriers in chickens and mice. This study aimed to investigate if PTAI could be used as an efficient carrier of a DNA vaccine. METHODS: Several vaccine mixtures were prepared by combining different proportions of the vaccine plasmid (carrying cDNA encoding a vaccine antigen, hemagglutinin from H5N1 influenza virus) and various compositions of PTAI. The vaccines were delivered by intramuscular injection to either chickens or mice. The presence of specific antibodies in sera collected from the immunized animals was analyzed by enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) test. RESULTS: The mixtures of PTAI with helper lipids, such as DOPE (1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine), DC-cholesterol [{3ß-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol} hydrochloride] or DOPC (1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) induced strong humoral response to the antigen encoded by the DNA vaccine plasmid. CONCLUSION: The animal immunization results confirmed that PTAI compositions, especially mixtures of PTAI with DOPE and DC-cholesterol, do work as effective carriers of DNA vaccines, comparable to the commercially available lipid transfection reagent.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Vacunación / Sistemas de Liberación de Medicamentos / Vacunas de ADN / Inmunidad Humoral Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Vacunación / Sistemas de Liberación de Medicamentos / Vacunas de ADN / Inmunidad Humoral Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido