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[Induction of apoptotic endonuclease EndoG with DNA-damaging agents initiates alternative splicing of telomerase catalytic subunit hTERT and inhibition of telomerase activity hTERT in human CD4+ and CD8+ T-lymphocytes]. / Induktsiia apoptoticheskoi éndonukleazy EndoG povrezhdaiushchimi DNK agentami vyzyvaet al'ternativnyi splaising kataliticheskoi sub"edinitsy telomerazy hTERT i ingibirovanie aktivnosti telomerazy v CD4+ I CD8+ T-limfotsitakh cheloveka.
Zhdanov, D D; Vasina, D A; Orlova, V S; Orlova, E V; Grishin, D V; Gladilina, Yu A; Pokrovskaya, M V; Aleksandrova, S S; Sokolov, N N.
Afiliación
  • Zhdanov DD; Institute of Biomedical Chemistry, Moscow, Russia.
  • Vasina DA; Peoples Friendship University of Russia, Moscow, Russia.
  • Orlova VS; Peoples Friendship University of Russia, Moscow, Russia.
  • Orlova EV; Institute of Theoretical and Experimental Biophysics, Moscow, Russia.
  • Grishin DV; Institute of Biomedical Chemistry, Moscow, Russia.
  • Gladilina YA; Institute of Biomedical Chemistry, Moscow, Russia.
  • Pokrovskaya MV; Institute of Biomedical Chemistry, Moscow, Russia.
  • Aleksandrova SS; Institute of Biomedical Chemistry, Moscow, Russia.
  • Sokolov NN; Institute of Biomedical Chemistry, Moscow, Russia.
Biomed Khim ; 63(4): 296-305, 2017 Jul.
Article en Ru | MEDLINE | ID: mdl-28862599
Activity of telomerase catalytic subunit hTERT (human Telomerase Reverse Transcriptase) can be regulated by alternative splicing of its mRNA. At present time exact mechanism of hTERT splicing is not fully understood. Apoptotic endonuclease EndoG is known to participate this process. EndoG expression is induced by DNA damages. The aim of this work was to investigate the ability of DNA-damaging agents with different mechanism of action to induce EndoG expression and inhibit telomerase activity due to the activation of hTERT alternative splicing in normal activated human CD4+ and CD8+ T-lymphocytes. All investigated DNA-damaging agents were able to induce EndoG expression. Cisplatin, a therapeutic compound, producing DNA cross-links induced the highest level of DNA damages and EndoG expression. Incubation of CD4+ and CD8+ T-cells with cisplatin caused the changes in proportion of hTERT splice variants and inhibition of telomerase activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño del ADN / Linfocitos T CD4-Positivos / Empalme Alternativo / Linfocitos T CD8-positivos / Telomerasa / Endodesoxirribonucleasas Límite: Humans Idioma: Ru Revista: Biomed Khim Año: 2017 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Rusia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño del ADN / Linfocitos T CD4-Positivos / Empalme Alternativo / Linfocitos T CD8-positivos / Telomerasa / Endodesoxirribonucleasas Límite: Humans Idioma: Ru Revista: Biomed Khim Año: 2017 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Rusia