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Long-term Functioning of Allogeneic Islets in Subcutaneous Tissue Pretreated With a Novel Cyclic Peptide Without Immunosuppressive Medication.
Kuwabara, Rei; Hamaguchi, Masahide; Fukuda, Takuya; Sakai, Hiroki; Inui, Makoto; Sakaguchi, Shimon; Iwata, Hiroo.
Afiliación
  • Kuwabara R; Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
  • Hamaguchi M; Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
  • Fukuda T; Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
  • Sakai H; Department of Endocrinology and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
  • Inui M; Department of Pharmacology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan.
  • Sakaguchi S; Department of Pharmacology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan.
  • Iwata H; Immunology Frontier Research Center, Osaka University, Osaka, Japan.
Transplantation ; 102(3): 417-425, 2018 03.
Article en En | MEDLINE | ID: mdl-28858989
BACKGROUND: There exists a need for a minimally invasive method of islet transplantation without immunosuppressive drugs for the treatment of type 1 diabetes. METHODS: In diabetic August Copenhagen Irish rats, an agarose rod containing the cyclic oligopeptide SEK-1005 (agarose-SEK rod) was implanted at 2 dorsal subcutaneous sites. Then these rods were removed, and 1500 islets of Langerhans isolated from Fischer 344 rats were transplanted into each of the pockets. RESULTS: Ten days after implantation of agarose-SEK rods, vascularized pockets were present. Nonfasting blood glucose levels confirmed long-term survival of the allogeneic islet grafts, without immunosuppressive therapy, in 8 of 10 recipients. Flow cytometry and gene expression analyses were performed to investigate the mechanisms underlying graft acceptance. Agarose-SEK rod implantation led to the formation of granulomatous tissue containing regulatory T cells that suppressed immune reactions against the allogeneic islet grafts. CONCLUSIONS: These results indicate that the use of an agarose-SEK rod to prevascularize a subcutaneous site may be a useful method for achieving successful allogeneic islet transplantation without immunosuppression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Tejido Subcutáneo / Inmunosupresores Límite: Animals Idioma: En Revista: Transplantation Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Cíclicos / Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Tejido Subcutáneo / Inmunosupresores Límite: Animals Idioma: En Revista: Transplantation Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos