Your browser doesn't support javascript.
loading
Genotoxic effects of high dose rate X-ray and low dose rate gamma radiation in ApcMin/+ mice.
Graupner, Anne; Eide, Dag M; Brede, Dag A; Ellender, Michele; Lindbo Hansen, Elisabeth; Oughton, Deborah H; Bouffler, Simon D; Brunborg, Gunnar; Olsen, Ann Karin.
Afiliación
  • Graupner A; Department of Molecular Biology, Norwegian Institute of Public Health, Oslo, 0403, Norway.
  • Eide DM; Centre for Environmental Radioactivity (CoE CERAD), Ås 1432, Norway.
  • Brede DA; Centre for Environmental Radioactivity (CoE CERAD), Ås 1432, Norway.
  • Ellender M; Department of Toxicology and Risk Assessment, Norwegian Institute of Public Health, Oslo, 0403, Norway.
  • Lindbo Hansen E; Centre for Environmental Radioactivity (CoE CERAD), Ås 1432, Norway.
  • Oughton DH; Department of Environmental Sciences, Norwegian University of Life Sciences, Ås 1432, Norway.
  • Bouffler SD; Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, Didcot, OX11 0RQ, England.
  • Brunborg G; Centre for Environmental Radioactivity (CoE CERAD), Ås 1432, Norway.
  • Olsen AK; Department of Research, Norwegian Radiation Protection Authority, Østerås, 1361, Norway.
Environ Mol Mutagen ; 58(8): 560-569, 2017 10.
Article en En | MEDLINE | ID: mdl-28856770
Risk estimates for radiation-induced cancer in humans are based on epidemiological data largely drawn from the Japanese atomic bomb survivor studies, which received an acute high dose rate (HDR) ionising radiation. Limited knowledge exists about the effects of chronic low dose rate (LDR) exposure, particularly with respect to the application of the dose and dose rate effectiveness factor. As part of a study to investigate the development of colon cancer following chronic LDR vs. acute HDR radiation, this study presents the results of genotoxic effects in blood of exposed mice. CBAB6 F1 Apc+/+ (wild type) and ApcMin/+ mice were chronically exposed to estimated whole body absorbed doses of 1.7 or 3.2 Gy 60 Co-γ-rays at a LDR (2.2 mGy h-1 ) or acutely exposed to 2.6 Gy HDR X-rays (1.3 Gy min-1 ). Genotoxic endpoints assessed in blood included chromosomal damage (flow cytometry based micronuclei (MN) assay), mutation analyses (Pig-a gene mutation assay), and levels of DNA lesions (Comet assay, single-strand breaks (ssb), alkali labile sites (als), oxidized DNA bases). Ionising radiation (ca. 3 Gy) induced genotoxic effects dependent on the dose rate. Chromosomal aberrations (MN assay) increased 3- and 10-fold after chronic LDR and acute HDR, respectively. Phenotypic mutation frequencies as well as DNA lesions (ssb/als) were modulated after acute HDR but not after chronic LDR. The ApcMin/+ genotype did not influence the outcome in any of the investigated endpoints. The results herein will add to the scant data available on genotoxic effects following chronic LDR of ionising radiation. Environ. Mol. Mutagen. 58:560-569, 2017. © 2017 The Authors Environmental and Molecular Mutagenesis published by Wiley Periodicals, Inc. on behalf of Environmental Mutagen Society.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño del ADN / Supervivencia Celular / Aberraciones Cromosómicas / Neoplasias Inducidas por Radiación Límite: Animals / Humans Idioma: En Revista: Environ Mol Mutagen Asunto de la revista: BIOLOGIA MOLECULAR / SAUDE AMBIENTAL Año: 2017 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño del ADN / Supervivencia Celular / Aberraciones Cromosómicas / Neoplasias Inducidas por Radiación Límite: Animals / Humans Idioma: En Revista: Environ Mol Mutagen Asunto de la revista: BIOLOGIA MOLECULAR / SAUDE AMBIENTAL Año: 2017 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Estados Unidos