Your browser doesn't support javascript.
loading
An in vitro model of azithromycin-induced persistent Chlamydia trachomatis infection.
Xue, Yaohua; Zheng, Heping; Mai, Zhida; Qin, Xiaolin; Chen, Wentao; Huang, Tao; Chen, Daxiang; Zheng, Lei.
Afiliación
  • Xue Y; Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, No. 1838 North Guangzhou Road, Guangzhou 510515, China.
  • Zheng H; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
  • Mai Z; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
  • Qin X; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
  • Chen W; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
  • Huang T; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
  • Chen D; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
  • Zheng L; Department of Research Center, Dermatology Hospital of Southern Medical University/Guangdong Provincial Dermatology Hospital, No. 2 Lujing Road, Guangzhou 510091, China.
FEMS Microbiol Lett ; 364(14)2017 08 01.
Article en En | MEDLINE | ID: mdl-28854672
Single-dose azithromycin is recommended for treating Chlamydia trachomatis infections. Here, we established an in vitro cell model of azithromycin-induced persistent infection. Azithromycin inhibited the replication of C. trachomatis in a dose-time-dependent manner. Electron microscopy indicated that small inclusions in the induced model contained enlarged, aberrant and non-infectious reticulate bodies. RT-PCR showed that C. trachomatis still has the ability to express the unprocessed 16S rRNA gene in the model and that C. trachomatis recovered after the removal of azithromycin with a peak recovery time of 24 h. The mutations in 23S rRNA, L4 and L22 genes were not found in persistent infection, and qRT-PCR analysis showed that the relative expression level of euo in azithromycin treated infection was upregulated while omcB was downregulated. In summary, this study provides a novel in vitro cell model to examine the characteristics of azithromycin-induced persistent infection and contribute to the development of treatments for C. trachomatis infection.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Chlamydia trachomatis / Técnicas Bacteriológicas / Azitromicina / Antibacterianos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: FEMS Microbiol Lett Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Chlamydia trachomatis / Técnicas Bacteriológicas / Azitromicina / Antibacterianos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: FEMS Microbiol Lett Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido