A theranostic dental pulp capping agent with improved MRI and CT contrast and biological properties.

Mastrogiacomo, S; Güvener, N; Dou, W; Alghamdi, H S; Camargo, W A; Cremers, J G O; Borm, P J A; Heerschap, A; Oosterwijk, E; Jansen, J A; Walboomers, X F

Mastrogiacomo, S; Güvener, N; Dou, W; Alghamdi, H S; Camargo, W A; Cremers, J G O; Borm, P J A; Heerschap, A; Oosterwijk, E; Jansen, J A; Walboomers, X F.

Afiliación

Mastrogiacomo S; Department of Biomaterials, Radboud University Medical Center, Nijmegen, The Netherlands.
Güvener N; Nano4Imaging GmbH, Pauwelsstrasse 17, Aachen, Germany; Institute for Experimental Molecular Imaging, RWTH Aachen University, Aachen, Germany.
Dou W; Department of Radiology and Nuclear Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Alghamdi HS; College of Dentistry Research Center, King Saud University, Riyadh, Saudi Arabia.
Camargo WA; Department of Biomaterials, Radboud University Medical Center, Nijmegen, The Netherlands.
Cremers JGO; Nano4Imaging GmbH, Pauwelsstrasse 17, Aachen, Germany.
Borm PJA; Nano4Imaging GmbH, Pauwelsstrasse 17, Aachen, Germany.
Heerschap A; Department of Radiology and Nuclear Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Oosterwijk E; Department of Urology, Radboud University Medical Center, Nijmegen, The Netherlands.
Jansen JA; Department of Biomaterials, Radboud University Medical Center, Nijmegen, The Netherlands.
Walboomers XF; Department of Biomaterials, Radboud University Medical Center, Nijmegen, The Netherlands. Electronic address: Frank.Walboomers@radboudumc.nl.

Acta Biomater ; 62: 340-351, 2017 10 15.

Article en En | MEDLINE | ID: mdl-28842333

RESUMEN

Different materials have been used for vital dental pulp treatment. Preferably a pulp capping agent should show appropriate biological performance, excellent handling properties, and a good imaging contrast. These features can be delivered into a single material through the combination of therapeutic and diagnostic agents (i.e. theranostic). Calcium phosphate based composites (CPCs) are potentially ideal candidate for pulp treatment, although poor imaging contrast and poor dentino-inductive properties are limiting their clinical use. In this study, a theranostic dental pulp capping agent was developed. First, imaging properties of the CPC were improved by using a core-shell structured dual contrast agent (csDCA) consisting of superparamagnetic iron oxide (SPIO) and colloidal gold, as MRI and CT contrast agent respectively. Second, biological properties were implemented by using a dentinogenic factor (i.e. bone morphogenetic protein 2, BMP-2). The obtained CPC/csDCA/BMP-2 composite was tested in vivo, as direct pulp capping agent, in a male Habsi goat incisor model. Our outcomes showed no relevant alteration of the handling and mechanical properties (e.g. setting time, injectability, and compressive strength) by the incorporation of csDCA particles. In vivo results proved MRI contrast enhancement up to 7weeks. Incisors treated with BMP-2 showed improved tertiary dentin deposition as well as faster cement degradation as measured by µCT assessment. In conclusion, the presented theranostic agent matches the imaging and regenerative requirements for pulp capping applications. STATEMENT OF SIGNIFICANCE: In this study, we combined diagnostic and therapeutic agents in order to developed a theranostic pulp capping agent with enhanced MRI and CT contrast and improved dentin regeneration ability. In our study we cover all the steps from material preparation, mechanical and in vitro characterization, to in vivo study in a goat dental model. To the best of our knowledge, this is the first time that a theranostic pulp capping material have been developed and tested in an in vivo animal model. Our promising results in term of imaging contrast enhancement and of induction of new dentin formation, open a new scenario in the development of innovative dental materials.

Asunto(s)

Resinas Acrílicas; Resinas Compuestas; Medios de Contraste; Incisivo; Imagen por Resonancia Magnética/métodos; Poliuretanos; Materiales de Recubrimiento Pulpar y Pulpectomía; Nanomedicina Teranóstica/métodos; Tomografía Computarizada por Rayos X/métodos; Resinas Acrílicas/química; Resinas Acrílicas/farmacocinética; Resinas Acrílicas/farmacología; Animales; Proteína Morfogenética Ósea 2/química; Proteína Morfogenética Ósea 2/farmacocinética; Proteína Morfogenética Ósea 2/farmacología; Resinas Compuestas/química; Resinas Compuestas/farmacocinética; Resinas Compuestas/farmacología; Medios de Contraste/química; Medios de Contraste/farmacocinética; Medios de Contraste/farmacología; Compuestos Férricos/química; Compuestos Férricos/farmacocinética; Compuestos Férricos/farmacología; Cabras; Oro Coloide/química; Oro Coloide/farmacocinética; Oro Coloide/farmacología; Humanos; Incisivo/diagnóstico por imagen; Incisivo/metabolismo; Incisivo/cirugía; Poliuretanos/química; Poliuretanos/farmacocinética; Poliuretanos/farmacología; Materiales de Recubrimiento Pulpar y Pulpectomía/química; Materiales de Recubrimiento Pulpar y Pulpectomía/farmacocinética; Materiales de Recubrimiento Pulpar y Pulpectomía/farmacología

Palabras clave

BMP-2 (bone morphogenetic protein-2); CPC (calcium phosphate cement); DCA (dual contrast agent); Direct pulp capping; MRI (magnetic resonance imaging)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliuretanos / Resinas Acrílicas / Imagen por Resonancia Magnética / Tomografía Computarizada por Rayos X / Resinas Compuestas / Medios de Contraste / Materiales de Recubrimiento Pulpar y Pulpectomía / Nanomedicina Teranóstica / Incisivo Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Acta Biomater Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido

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