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Characterization of microbiota in Arapaima gigas intestine and isolation of potential probiotic bacteria.
do Vale Pereira, G; da Cunha, D G; Pedreira Mourino, J L; Rodiles, A; Jaramillo-Torres, A; Merrifield, D L.
Afiliación
  • do Vale Pereira G; Aquatic Animal Nutrition and Health Research Group, School of Biological and Marine Sciences, Plymouth University, Plymouth, UK.
  • da Cunha DG; CAPES Foundation, Ministry of Education of Brazil, Brazilia-DF, Brazil.
  • Pedreira Mourino JL; Mar e Terra Ind. Com. de pescados S/A, Rondonia, Brazil.
  • Rodiles A; Aquaculture Department, Federal University of Santa Catarina, Florianopolis, Brazil.
  • Jaramillo-Torres A; Aquatic Animal Nutrition and Health Research Group, School of Biological and Marine Sciences, Plymouth University, Plymouth, UK.
  • Merrifield DL; Aquatic Animal Nutrition and Health Research Group, School of Biological and Marine Sciences, Plymouth University, Plymouth, UK.
J Appl Microbiol ; 123(5): 1298-1311, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28833934
AIMS: The aim of this study was to determine the intestinal microbiota of pirarucu (Arapaima gigas) in different growth stages (adult and fingerlings) and to isolate and identify potential probiotic bacteria. METHODS AND RESULTS: High-throughput sequencing analysis of the intestinal contents revealed that the majority of sequences belonged to the Proteobacteria, Fusobacteria and Firmicutes phyla. At the genus level, the greatest number of sequences belonged to Bradyrhizobium in adult fish, while Cetobacterium was the most abundant in juvenile fish. Twenty-three lactic-acid bacteria (LABs) were isolated on MRS agar from healthy juvenile fish. The isolates were tested in vitro for probiotic properties. Two isolates (identified as strains of Lactococcus lactis subsp. lactis and Enterococcus faecium) displayed antagonism against all 10 pathogens tested, were nonhaemolytic and maintained good viability for at least 3 weeks when supplemented to fish diets. The presence of a number of antibiotic resistance genes (ARGs), conferring resistance to erythromycin, tetracycline and chloramphenicol, was investigated by PCR. CONCLUSIONS: The absence of ARGs investigated the potential to antagonize pathogens, and favourable growth and survival characteristics indicate that these autochthonous isolates have the potential to be considered probiotics, which will be studied in future in vivo experiments. SIGNIFICANCE AND IMPACT OF THE STUDY: This study has demonstrated, for the first time, the normal microbiota in the A. gigas intestine during different life stages and the presence of LAB strains. It also demonstrated LAB antibiotic resistance and antagonistic behaviour against pathogens isolated from the same fish.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Probióticos / Lactobacillales / Peces / Microbioma Gastrointestinal / Intestinos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Probióticos / Lactobacillales / Peces / Microbioma Gastrointestinal / Intestinos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido