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Cisplatin-induced human peripheral blood mononuclear cells' oxidative stress and nephrotoxicity in head and neck cancer patients: the influence of hydrogen peroxide.
Quintanilha, Júlia C F; Visacri, Marília B; Sousa, Vanessa M; Bastos, Larissa B; Vaz, Camila O; Guarnieri, João P O; Amaral, Laís S; Malaguti, Carina; Lima, Carmen S P; Vercesi, Anibal E; Moriel, Patricia.
Afiliación
  • Quintanilha JCF; School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Visacri MB; School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Sousa VM; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), 200 Cândido Portinari, Campinas, SP, 13083-871, Brazil.
  • Bastos LB; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), 200 Cândido Portinari, Campinas, SP, 13083-871, Brazil.
  • Vaz CO; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), 200 Cândido Portinari, Campinas, SP, 13083-871, Brazil.
  • Guarnieri JPO; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), 200 Cândido Portinari, Campinas, SP, 13083-871, Brazil.
  • Amaral LS; School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Malaguti C; School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Lima CSP; School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Vercesi AE; School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Moriel P; Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), 200 Cândido Portinari, Campinas, SP, 13083-871, Brazil. patricia.moriel@fcf.unicamp.br.
Mol Cell Biochem ; 440(1-2): 139-145, 2018 Mar.
Article en En | MEDLINE | ID: mdl-28828710
Cisplatin is a widely used antineoplastic agent in the treatment of head and neck cancer. However, it is highly nephrotoxic. Oxidative stress is the main mechanism responsible for cisplatin-induced nephrotoxicity. The aim of this study was to characterize cisplatin-induced nephrotoxicity, oxidative stress in peripheral blood mononuclear cells, and the relationship between them. Twenty-four patients were included in the study. Patients had their blood collected prior to cisplatin administration, and 5 and 20 days after initiating therapy, to assess renal function and to determine oxidative stress with MitoSOX™Red, H2DCF-DA, and Amplex® Red tests. Renal function was assessed by measuring serum creatinine, creatinine clearance, and blood urea nitrogen (BUN). Serum creatinine and creatinine clearance were used to grade nephrotoxicity using Common Terminology Criteria for Adverse Events (CTCAE) v4.0. Compared to baseline values, the mean BUN and serum creatinine increased 135 and 100%, respectively, 5 days after cisplatin infusion. Mean creatinine clearance showed a 43% decrease compared to baseline value. Non-statistically significant changes in superoxide anion (O 2•- ), hydrogen peroxide (H2O2), and general reactive oxygen species production occurred. A higher production of H2O2 was correlated with variation in serum creatinine, and was associated with higher grades for serum creatinine increases and creatinine clearance reductions. Linear regression analyses showed an association between H2O2 production and serum creatinine, creatinine clearance, and BUN levels. These results were observed for 5 days following cisplatin administration. In conclusion, H2O2 production was significantly related to changes in all renal parameters that were evaluated, following the cisplatin infusion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Cisplatino / Estrés Oxidativo / Neoplasias de Cabeza y Cuello / Peróxido de Hidrógeno / Enfermedades Renales Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Mol Cell Biochem Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Cisplatino / Estrés Oxidativo / Neoplasias de Cabeza y Cuello / Peróxido de Hidrógeno / Enfermedades Renales Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Mol Cell Biochem Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos