Your browser doesn't support javascript.
loading
Two separate pathways regulate protein stability of ATM/ATR-related protein kinases Mec1 and Tel1 in budding yeast.
Goto, Greicy H; Ogi, Hiroo; Biswas, Himadri; Ghosh, Avik; Tanaka, Seiji; Sugimoto, Katsunori.
Afiliación
  • Goto GH; Department of Microbiology, Biochemistry and Molecular Genetics, International Center for Public Health, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, United States of America.
  • Ogi H; Department of Microbiology, Biochemistry and Molecular Genetics, International Center for Public Health, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, United States of America.
  • Biswas H; Department of Microbiology, Biochemistry and Molecular Genetics, International Center for Public Health, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, United States of America.
  • Ghosh A; Department of Microbiology, Biochemistry and Molecular Genetics, International Center for Public Health, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, United States of America.
  • Tanaka S; Division of Microbial Genetics, National Institute of Genetics, and Department of Genetics, School of Life Science, Graduate School for Advanced Studies, (SOKENDAI), Mishima, Shizuoka, Japan.
  • Sugimoto K; Department of Microbiology, Biochemistry and Molecular Genetics, International Center for Public Health, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, United States of America.
PLoS Genet ; 13(8): e1006873, 2017 Aug.
Article en En | MEDLINE | ID: mdl-28827813
Checkpoint signaling requires two conserved phosphatidylinositol 3-kinase-related protein kinases (PIKKs): ATM and ATR. In budding yeast, Tel1 and Mec1 correspond to ATM and ATR, respectively. The Tel2-Tti1-Tti2 (TTT) complex connects to the Rvb1-Rvb2-Tah1-Pih1 (R2TP) complex for the protein stability of PIKKs; however, TTT-R2TP interaction only partially mediates ATM and ATR protein stabilization. How TTT controls protein stability of ATM and ATR remains to be precisely determined. Here we show that Asa1, like Tel2, plays a major role in stabilization of newly synthesized Mec1 and Tel1 proteins whereas Pih1 contributes to Mec1 and Tel1 stability at high temperatures. Although Asa1 and Pih1 both interact with Tel2, no Asa1-Pih1 interaction is detected. Pih1 is distributed in both the cytoplasm and nucleus wheres Asa1 localizes largely in the cytoplasm. Asa1 and Pih1 are required for proper DNA damage checkpoint signaling. Our findings provide a model in which two different Tel2 pathways promote protein stabilization of Mec1 and Tel1 in budding yeast.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Serina-Treonina Quinasas / Proteínas de Saccharomyces cerevisiae / Proteínas de Unión a Telómeros / Péptidos y Proteínas de Señalización Intracelular / Proteínas de la Ataxia Telangiectasia Mutada Tipo de estudio: Prognostic_studies Idioma: En Revista: PLoS Genet Asunto de la revista: GENETICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Serina-Treonina Quinasas / Proteínas de Saccharomyces cerevisiae / Proteínas de Unión a Telómeros / Péptidos y Proteínas de Señalización Intracelular / Proteínas de la Ataxia Telangiectasia Mutada Tipo de estudio: Prognostic_studies Idioma: En Revista: PLoS Genet Asunto de la revista: GENETICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos