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Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios.
Winter, Ethan S; Schwarz, Anna; Fabig, Gunar; Feldman, Jessica L; Pires-daSilva, André; Müller-Reichert, Thomas; Sadler, Penny L; Shakes, Diane C.
Afiliación
  • Winter ES; Department of Biology, College of William and Mary, Williamsburg, VA 23187, USA.
  • Schwarz A; Experimental Center, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, 01307, Germany.
  • Fabig G; Experimental Center, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, 01307, Germany.
  • Feldman JL; Department of Biology, Stanford University, Stanford, CA, 94305, USA.
  • Pires-daSilva A; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
  • Müller-Reichert T; Experimental Center, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, 01307, Germany.
  • Sadler PL; Department of Biology, College of William and Mary, Williamsburg, VA 23187, USA.
  • Shakes DC; Department of Biology & Biochemistry, University of Houston, Houston, TX, 77204, USA.
Development ; 144(18): 3253-3263, 2017 09 15.
Article en En | MEDLINE | ID: mdl-28827395
Asymmetric partitioning is an essential component of many developmental processes. As spermatogenesis concludes, sperm are streamlined by discarding unnecessary cellular components into cellular wastebags called residual bodies (RBs). During nematode spermatogenesis, this asymmetric partitioning event occurs shortly after anaphase II, and both microtubules and actin partition into a central RB. Here, we use fluorescence and transmission electron microscopy to elucidate and compare the intermediate steps of RB formation in Caenorhabditis elegans, Rhabditis sp. SB347 (recently named Auanema rhodensis) and related nematodes. In all cases, intact microtubules reorganize and move from centrosomal to non-centrosomal sites at the RB-sperm boundary whereas actin reorganizes through cortical ring expansion and clearance from the poles. However, in species with tiny spermatocytes, these cytoskeletal changes are restricted to one pole. Consequently, partitioning yields one functional sperm with the X-bearing chromosome complement and an RB with the other chromosome set. Unipolar partitioning may not require an unpaired X, as it also occurs in XX spermatocytes. Instead, constraints related to spermatocyte downsizing may have contributed to the evolution of a sperm cell equivalent to female polar bodies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Razón de Masculinidad / Espermatozoides / Citoesqueleto / Caenorhabditis elegans / Tamaño de la Célula / División Celular Asimétrica Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Razón de Masculinidad / Espermatozoides / Citoesqueleto / Caenorhabditis elegans / Tamaño de la Célula / División Celular Asimétrica Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido