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Maternal expression of the histone demethylase Kdm4a is crucial for pre-implantation development.
Sankar, Aditya; Kooistra, Susanne Marije; Gonzalez, Javier Martin; Ohlsson, Claes; Poutanen, Matti; Helin, Kristian.
Afiliación
  • Sankar A; Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen 2200, Denmark.
  • Kooistra SM; Centre for Epigenetics, University of Copenhagen, Copenhagen 2200, Denmark.
  • Gonzalez JM; The Danish Stem Cell Center (Danstem), Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.
  • Ohlsson C; Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen 2200, Denmark.
  • Poutanen M; Centre for Epigenetics, University of Copenhagen, Copenhagen 2200, Denmark.
  • Helin K; Core Facility for Transgenic Mice, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Development ; 144(18): 3264-3277, 2017 09 15.
Article en En | MEDLINE | ID: mdl-28827393
Regulation of chromatin composition through post-translational modifications of histones contributes to transcriptional regulation and is essential for many cellular processes, including differentiation and development. KDM4A (JMJD2A) is a lysine demethylase with specificity towards di- and tri-methylated lysine 9 and lysine 36 of histone H3 (H3K9me2/me3 and H3K36me2/me3). Here, we report that Kdm4a as a maternal factor plays a key role in embryo survival and is vital for female fertility. Kdm4a-/- female mice ovulate normally with comparable fertilization but poor implantation rates, and cannot support healthy transplanted embryos to term. This is due to a role for Kdm4a in uterine function, where its loss causes reduced expression of key genes involved in ion transport, nutrient supply and cytokine signalling, which impact embryo survival. In addition, a significant proportion of Kdm4a-deficient oocytes displays a poor intrinsic ability to develop into blastocysts. These embryos cannot compete with healthy embryos for implantation in vivo, highlighting Kdm4a as a maternal effect gene. Thus, our study dissects an important dual role for maternal Kdm4a in determining faithful early embryonic development and the implantation process.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Implantación del Embrión / Histona Demetilasas Límite: Animals / Pregnancy Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Implantación del Embrión / Histona Demetilasas Límite: Animals / Pregnancy Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido