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Bioinformatics for NGS-based metagenomics and the application to biogas research.
Jünemann, Sebastian; Kleinbölting, Nils; Jaenicke, Sebastian; Henke, Christian; Hassa, Julia; Nelkner, Johanna; Stolze, Yvonne; Albaum, Stefan P; Schlüter, Andreas; Goesmann, Alexander; Sczyrba, Alexander; Stoye, Jens.
Afiliación
  • Jünemann S; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany; Faculty of Technology, Bielefeld University, Bielefeld, Germany. Electronic address: jueneman@cebitec.uni-bielefeld.de.
  • Kleinbölting N; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Jaenicke S; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany; Bioinformatics and Systems Biology, Justus-Liebig-Universität, Gießen, Germany.
  • Henke C; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Hassa J; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Nelkner J; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Stolze Y; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Albaum SP; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Schlüter A; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.
  • Goesmann A; Bioinformatics and Systems Biology, Justus-Liebig-Universität, Gießen, Germany.
  • Sczyrba A; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany; Faculty of Technology, Bielefeld University, Bielefeld, Germany.
  • Stoye J; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany; Faculty of Technology, Bielefeld University, Bielefeld, Germany.
J Biotechnol ; 261: 10-23, 2017 Nov 10.
Article en En | MEDLINE | ID: mdl-28823476
Metagenomics has proven to be one of the most important research fields for microbial ecology during the last decade. Starting from 16S rRNA marker gene analysis for the characterization of community compositions to whole metagenome shotgun sequencing which additionally allows for functional analysis, metagenomics has been applied in a wide spectrum of research areas. The cost reduction paired with the increase in the amount of data due to the advent of next-generation sequencing led to a rapidly growing demand for bioinformatic software in metagenomics. By now, a large number of tools that can be used to analyze metagenomic datasets has been developed. The Bielefeld-Gießen center for microbial bioinformatics as part of the German Network for Bioinformatics Infrastructure bundles and imparts expert knowledge in the analysis of metagenomic datasets, especially in research on microbial communities involved in anaerobic digestion residing in biogas reactors. In this review, we give an overview of the field of metagenomics, introduce into important bioinformatic tools and possible workflows, accompanied by application examples of biogas surveys successfully conducted at the Center for Biotechnology of Bielefeld University.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biología Computacional / Metagenoma / Biocombustibles / Metagenómica / Secuenciación de Nucleótidos de Alto Rendimiento Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biología Computacional / Metagenoma / Biocombustibles / Metagenómica / Secuenciación de Nucleótidos de Alto Rendimiento Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos