Your browser doesn't support javascript.
loading
Musashi-1 Enhances Glioblastoma Cell Migration and Cytoskeletal Dynamics through Translational Inhibition of Tensin3.
Chen, Hsiao-Yun; Lin, Liang-Ting; Wang, Mong-Lien; Laurent, Benoit; Hsu, Chih-Hung; Pan, Chih-Ming; Jiang, Wan-Ru; Chen, Pau-Yuan; Ma, Hsin-I; Chen, Yi-Wei; Huang, Pin-I; Chiou, Arthur; Chiou, Shih-Hwa.
Afiliación
  • Chen HY; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Lin LT; Stem Cell Center, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Wang ML; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Laurent B; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong.
  • Hsu CH; Stem Cell Center, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Pan CM; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan.
  • Jiang WR; Boston Children Hospital and Harvard Medical School, Boston, MA, USA.
  • Chen PY; Boston Children Hospital and Harvard Medical School, Boston, MA, USA.
  • Ma HI; Program in Epigenetic and Molecular Cell Biology, School of Medicine and Public Health, Zhejiang University, Hangzhou, China.
  • Chen YW; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan.
  • Huang PI; Center for Cell Therapy, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
  • Chiou A; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Chiou SH; Institute of Biomedical Engineering, National Chiao-Tung University, Hsinchu, Taiwan.
Sci Rep ; 7(1): 8710, 2017 08 18.
Article en En | MEDLINE | ID: mdl-28821879
The RNA-binding protein Musashi-1 (MSI1) exerts essential roles in multiple cellular functions, such as maintenance of self-renewal and pluripotency of stem cells. MSI1 overexpression has been observed in several tumor tissues, including glioblastoma (GBM), and is considered as a well-established marker for tumor metastasis and recurrence. However, the molecular mechanisms by which MSI1 regulates cell migration are still undetermined. Here we reported that MSI1 alters cell morphology, promotes cell migration, and increases viscoelasticity of GBM cells. We also found that MSI1 directly binds to the 3'UTR of Tensin 3 (TNS3) mRNA, a negative regulator of cell migration, to inhibit its translation. Additionally, we identified that RhoA-GTP could be a potential regulator in MSI1/TNS3-mediated cell migration and morphological changes. In a xenograft animal model, high expression ratio of MSI1 to TNS3 enhanced GBM tumor migration. We also confirmed that MSI1 and TNS3 expressions are mutually exclusive in migratory tumor lesions, and GBM patients with MSI1high/TNS3low pattern tend to have poor clinical outcome. Taken together, our findings suggested a critical role of MSI1-TNS3 axis in regulating GBM migration and highlighted that the ratio of MSI1/TNS3 could predict metastatic and survival outcome of GBM patients.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biosíntesis de Proteínas / Citoesqueleto / Movimiento Celular / Proteínas de Unión al ARN / Glioblastoma / Tensinas / Proteínas del Tejido Nervioso Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biosíntesis de Proteínas / Citoesqueleto / Movimiento Celular / Proteínas de Unión al ARN / Glioblastoma / Tensinas / Proteínas del Tejido Nervioso Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido