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Oleic acid Enhances Dengue Virus But Not Dengue Virus-Like Particle Production from Mammalian Cells.
Ramphan, Suwipa; Suksathan, Sathiporn; Wikan, Nitwara; Ounjai, Puey; Boonthaworn, Kanpong; Rimthong, Poramate; Kanjanapruthipong, Tapanee; Worawichawong, Suchin; Jongkaewwattana, Anan; Wongsiriroj, Nuttaporn; Smith, Duncan R.
Afiliación
  • Ramphan S; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, 73170, Thailand.
  • Suksathan S; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, 73170, Thailand.
  • Wikan N; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, 73170, Thailand.
  • Ounjai P; Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
  • Boonthaworn K; Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
  • Rimthong P; Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
  • Kanjanapruthipong T; Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
  • Worawichawong S; Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
  • Jongkaewwattana A; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
  • Wongsiriroj N; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, 73170, Thailand.
  • Smith DR; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, 73170, Thailand. duncan.smi@mahidol.ac.th.
Mol Biotechnol ; 59(9-10): 385-393, 2017 Oct.
Article en En | MEDLINE | ID: mdl-28791613
Despite the recent introduction of a commercial vaccine, the mosquito-transmitted dengue virus is still a worldwide public health problem. Based on the live attenuated vaccine strategy, the commercial vaccine has a less than optimal protective profile. Virus-like particles (VLPs) offer an attractive alternate vaccination strategy due to the effectively native presentation of epitopes in the absence of any infectious genetic material. However, the production of amounts of VLP in a platform that can support commercial development remains a major obstacle. This study generated two DENV 2 VLPs [codon-optimized and chimeric DENV/Japanese encephalitis virus (JEV)] and directly compared yields of these constructs by western blotting and dot blot hybridization. The effect of oleic acid supplementation, a process known to increase DENV production in natural infection, was also investigated. Results showed that the chimeric construct gave a two- to threefold higher yield than the codon-optimized construct and that while oleic acid increased DENV virion production in natural infection, it inhibited VLP production. These results suggest that further optimization of DENV VLP expression is possible, but it will require more understanding of how native DENV infection remodels the host cell machinery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Oléico / Dengue / Virus del Dengue / Vacunas de Partículas Similares a Virus Límite: Animals / Humans Idioma: En Revista: Mol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Oléico / Dengue / Virus del Dengue / Vacunas de Partículas Similares a Virus Límite: Animals / Humans Idioma: En Revista: Mol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza