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Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange.
Browning, K L; Lind, T K; Maric, S; Malekkhaiat-Häffner, S; Fredrikson, G N; Bengtsson, E; Malmsten, M; Cárdenas, M.
Afiliación
  • Browning KL; Department of Pharmacy, Uppsala University, Uppsala, Sweden.
  • Lind TK; Department of Biomedical Sciences and Biofilms, Malmö University, Malmö, Sweden.
  • Maric S; Department of Biomedical Sciences and Biofilms, Malmö University, Malmö, Sweden.
  • Malekkhaiat-Häffner S; Department of Pharmacy, Uppsala University, Uppsala, Sweden.
  • Fredrikson GN; Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden.
  • Bengtsson E; Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden.
  • Malmsten M; Department of Pharmacy, Uppsala University, Uppsala, Sweden. martin.malmsten@farmaci.uu.se.
  • Cárdenas M; Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark. martin.malmsten@farmaci.uu.se.
Sci Rep ; 7(1): 7478, 2017 08 07.
Article en En | MEDLINE | ID: mdl-28785025
High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the 'good' cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the 'bad' cholesterol, depositing lipid material into the vascular wall.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Membrana Celular / Membrana Dobles de Lípidos / Lipoproteínas Tipo de estudio: Etiology_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Membrana Celular / Membrana Dobles de Lípidos / Lipoproteínas Tipo de estudio: Etiology_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido