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Quantification of egg ovalbumin hydrolysate-derived anti-hypertensive peptides in an in vitro model combining luminal digestion with intestinal Caco-2 cell transport.
Grootaert, Charlotte; Jacobs, Griet; Matthijs, Bea; Pitart, Judit; Baggerman, Geert; Possemiers, Sam; Van der Saag, Hans; Smagghe, Guy; Van Camp, John; Voorspoels, Stefan.
Afiliación
  • Grootaert C; Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent, Belgium.
  • Jacobs G; Flemish Institute for Technological Research (VITO NV), Mol, Belgium.
  • Matthijs B; Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent, Belgium.
  • Pitart J; Prodigest, Ghent, Belgium.
  • Baggerman G; Flemish Institute for Technological Research (VITO NV), Mol, Belgium; Center for Proteomics, University of Antwerp, Antwerp, Belgium.
  • Possemiers S; Prodigest, Ghent, Belgium; Bioactor BV, Maastricht, The Netherlands.
  • Van der Saag H; Bioactor BV, Maastricht, The Netherlands.
  • Smagghe G; Department of Crop Protection, Ghent University, Ghent, Belgium.
  • Van Camp J; Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent, Belgium. Electronic address: john.vancamp@ugent.be.
  • Voorspoels S; Flemish Institute for Technological Research (VITO NV), Mol, Belgium.
Food Res Int ; 99(Pt 1): 531-541, 2017 09.
Article en En | MEDLINE | ID: mdl-28784514
Food-derived peptides can impact blood pressure through several mechanisms. However, their fate in the gastro-intestinal tract and bioavailability are difficult to assess because of their fast degradation and challenging analysis in physiologically relevant matrices. The aim of this study was to construct an in vitro bioavailability methodology in which luminal digestion is combined with Caco-2 cell transport. Egg ovalbumin hydrolysate, both in pure form and mixed with a food matrix, was used as a test case. Results indicate that a food matrix protected bioactive peptides from luminal digestion, especially in small intestine. Moreover, the Caco-2 absorption peak was extended over a longer time period (>60min) compared to the pure peptide solutions (~15min) which in total resulted in a 3-12 times higher absorption of the bioactive sequences after 60min compared to fasted conditions. These results suggest further investigation is warranted towards peptide-based functional foods with improved gastro-intestinal stability and longer-term release in the blood.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Ovalbúmina / Digestión / Absorción Intestinal / Mucosa Intestinal / Antihipertensivos Límite: Humans Idioma: En Revista: Food Res Int Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Canadá
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Ovalbúmina / Digestión / Absorción Intestinal / Mucosa Intestinal / Antihipertensivos Límite: Humans Idioma: En Revista: Food Res Int Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Canadá