Allele frequency distribution of two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity.
Am J Hum Genet
; 39(4): 489-501, 1986 Oct.
Article
en En
| MEDLINE
| ID: mdl-2876630
The allele frequency distribution of two highly polymorphic DNA sequences has been determined in three ethnic groups (American blacks, Caucasoids, and Hispanics) from the New York metropolitan area. The two loci examined were D14S1 and the flanking region of HRAS-1. The former was analyzed in EcoRI-digested DNA and the latter in TaqI-digested DNA. Approximately 700 DNA samples from unrelated individuals were digested with each of these enzymes and hybridized with the appropriate recombinant DNA probes. The size range of the DNA fragments detected for the D14S1 polymorphism varied from 14.3 to 32.5 kilobase pairs (kbp). The number of alleles identified under the experimental conditions used in this study was more than 40. For the HRAS-1 polymorphism, we have detected 18 different alleles varying in size from 1.85 to 4.5 kbp. Although the number of alleles observed in the different ethnic groups examined was very similar, the relative frequency of them varied significantly. The results presented here can be used as the basis for the utilization of DNA RFLP for the purpose of identity, such as paternity determinations or the analysis of forensic material. As an example, we have compared the results of paternity cases analyzed by HLA typing with those obtained with these two DNA polymorphisms. The values of paternity index and power of exclusion were similar by both procedures.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paternidad
/
Polimorfismo Genético
/
Polimorfismo de Longitud del Fragmento de Restricción
/
ADN
/
Etnicidad
/
Alelos
Límite:
Humans
País/Región como asunto:
America do norte
Idioma:
En
Revista:
Am J Hum Genet
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Estados Unidos