Purification and properties of an induced beta-D-glucosidase from stachybotrys atra.

de Gussem, R L; Aerts, G M; Claeyssens, M; de Bruyne, C K

de Gussem, R L; Aerts, G M; Claeyssens, M; de Bruyne, C K.

Biochim Biophys Acta ; 525(1): 142-53, 1978 Jul 07.

Article en En | MEDLINE | ID: mdl-28763

RESUMEN

We have purified an induced beta-D-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) from Stachybotrys atra to apparent homogeneity. The induced enzyme was clearly different from the constitutive beta-D-glucosidase. The molecular weight was 65 500-69 000, the pH optimum was at 6.7 and the isoelectric point at 4.8. Carbohydrate content (related to glucose) was 14.4%. The enzyme showed beta-D-glucosidase, beta-D-xylosidase and beta-D-thioglucosidase activity. These three activities sppear to be due to the same enzyme. The enzyme was strongly inhibited by D-glucono-(1 leads to 5)-lactone and nojirimycin and was very sensitive to sulfhydryl reagents.

Asunto(s)

Glucosidasas/metabolismo; Hongos Mitospóricos/enzimología; Stachybotrys/enzimología; beta-Glucosidasa/metabolismo; 1-Desoxinojirimicina/análogos & derivados; Antibacterianos/farmacología; Ácido Ditionitrobenzoico/farmacología; Inducción Enzimática; Gluconatos/farmacología; Glucosamina/farmacología; Hexosas/análisis; Concentración de Iones de Hidrógeno; Cinética; Peso Molecular; Estereoisomerismo; Especificidad por Sustrato; Temperatura; beta-Glucosidasa/aislamiento & purificación

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Stachybotrys / Beta-Glucosidasa / Hongos Mitospóricos / Glucosidasas Idioma: En Revista: Biochim Biophys Acta Año: 1978 Tipo del documento: Article Pais de publicación: Países Bajos

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