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Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus.
Vancoillie, Leen; Hebberecht, Laura; Dauwe, Kenny; Demecheleer, Els; Dinakis, Sylvie; Vaneechoutte, Dries; Mortier, Virginie; Verhofstede, Chris.
Afiliación
  • Vancoillie L; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
  • Hebberecht L; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
  • Dauwe K; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
  • Demecheleer E; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
  • Dinakis S; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
  • Vaneechoutte D; VIB Department of Plant Systems Biology, Ghent University, Ghent, Belgium.
  • Mortier V; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium.
  • Verhofstede C; Aids Reference Laboratory, Department of Clinical Biology, Microbiology and Immunology, Ghent University, Ghent, Belgium. Electronic address: chris.verhofstede@ugent.be.
Virology ; 510: 185-193, 2017 10.
Article en En | MEDLINE | ID: mdl-28750322
HIV-infected patients on antiretroviral therapy (ART) may present low-level viremia (LLV) above the detection level of current viral load assays. In many cases LLV is persistent but does not result in overt treatment failure or selection of drug resistant viral variants. To elucidate whether LLV reflects active virus replication, we extensively sequenced pol and env genes of the viral populations present before and during LLV in 18 patients and searched for indications of genetic evolution. Maximum likelihood phylogenetic trees were inspected for temporal structure both visually and by linear regression analysis of root-to-tip and pairwise distances. Viral coreceptor tropism was assessed at different time points before and during LLV. In none of the patients consistent indications for genetic evolution were found over a median period of 4.8 years of LLV. As such these findings could not provide evidence that active virus replication is the main driver of LLV.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 / Evolución Molecular / Antirretrovirales Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: Virology Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 / Evolución Molecular / Antirretrovirales Tipo de estudio: Observational_studies Límite: Humans Idioma: En Revista: Virology Año: 2017 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos