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Lithium Sensitive ORAI1 Expression, Store Operated Ca2+ Entry and Suicidal Death of Neurons in Chorea-Acanthocytosis.
Pelzl, Lisann; Hauser, Stefan; Elsir, Bhaeldin; Sukkar, Basma; Sahu, Itishri; Singh, Yogesh; Höflinger, Philip; Bissinger, Rosi; Jemaà, Mohamed; Stournaras, Christos; Schöls, Ludger; Lang, Florian.
Afiliación
  • Pelzl L; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Hauser S; German Center for Neurodegenerative Diseases, Tübingen, Germany.
  • Elsir B; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Sukkar B; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Sahu I; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Singh Y; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Höflinger P; Department of Neurology and Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany.
  • Bissinger R; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Jemaà M; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Stournaras C; Department of Physiology I, University of Tübingen, Tübingen, Germany.
  • Schöls L; Department of Biochemistry, University of Crete Medical School, Heraklion, Greece.
  • Lang F; German Center for Neurodegenerative Diseases, Tübingen, Germany.
Sci Rep ; 7(1): 6457, 2017 07 25.
Article en En | MEDLINE | ID: mdl-28743945
Chorea-Acanthocytosis (ChAc), a neurodegenerative disorder, results from loss-of-function-mutations of chorein-encoding gene VPS13A. In tumour cells chorein up-regulates ORAI1, a Ca2+-channel accomplishing store operated Ca2+-entry (SOCE) upon stimulation by STIM1. Furthermore SOCE could be up-regulated by lithium. The present study explored whether SOCE impacts on neuron apoptosis. Cortical neurons were differentiated from induced pluripotent stem cells generated from fibroblasts of ChAc patients and healthy volunteers. ORAI1 and STIM1 transcript levels and protein abundance were estimated from qRT-PCR and Western blotting, respectively, cytosolic Ca2+-activity ([Ca2+]i) from Fura-2-fluorescence, as well as apoptosis from annexin-V-binding and propidium-iodide uptake determined by flow cytometry. As a result, ORAI1 and STIM1 transcript levels and protein abundance and SOCE were significantly smaller and the percentage apoptotic cells significantly higher in ChAc neurons than in control neurons. Lithium treatment (2 mM, 24 hours) increased significantly ORAI1 and STIM1 transcript levels and protein abundance, an effect reversed by inhibition of Serum & Glucocorticoid inducible Kinase 1. ORAI1 blocker 2-APB (50 µM, 24 hours) significantly decreased SOCE, markedly increased apoptosis and abrogated the anti-apoptotic effect of lithium. In conclusion, enhanced neuronal apoptosis in ChAc at least partially results from decreased ORAI1 expression and SOCE, which could be reversed by lithium treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Neuroacantocitosis / Proteína ORAI1 / Litio / Neuronas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Neuroacantocitosis / Proteína ORAI1 / Litio / Neuronas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido