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Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats.
D'Arpa, Salvatore; Zabbia, Giovanni; Cannizzaro, Carla; Salimbeni, Grazia; Plescia, Fulvio; Mariolo, Alessio Vincenzo; Cassata, Giovanni; Cicero, Luca; Puleio, Roberto; Martorana, Anna; Moschella, Francesco; Cordova, Adriana.
Afiliación
  • D'Arpa S; a Plastische Heelkunde , Universitair Ziekenhuis Gent , Gent , Belgium.
  • Zabbia G; b Division of Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences , University of Palermo , Palermo , Italy.
  • Cannizzaro C; c Department of Sciences for Health Promotion and Mother and Child Care 'GIUSEPPE D'ALESSANDRO' , University of Palermo , Palermo , Italy.
  • Salimbeni G; d S. Rossore Hospital , Pisa , Italy.
  • Plescia F; c Department of Sciences for Health Promotion and Mother and Child Care 'GIUSEPPE D'ALESSANDRO' , University of Palermo , Palermo , Italy.
  • Mariolo AV; b Division of Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences , University of Palermo , Palermo , Italy.
  • Cassata G; e Laboratory Animal House/Unit , Institute of Experimental Zooprophylactic of Sicily , Palermo , Italy.
  • Cicero L; e Laboratory Animal House/Unit , Institute of Experimental Zooprophylactic of Sicily , Palermo , Italy.
  • Puleio R; f Histopathology and Immunohistochemistry Laboratory , Institute Experimental Zooprophylactic of Sicily , Palermo , Italy.
  • Martorana A; g Department of Human Pathology , University of Palermo , Palermo , Italy.
  • Moschella F; b Division of Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences , University of Palermo , Palermo , Italy.
  • Cordova A; b Division of Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences , University of Palermo , Palermo , Italy.
Acta Chir Belg ; 118(1): 27-35, 2018 Feb.
Article en En | MEDLINE | ID: mdl-28738725
BACKGROUND: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. METHODS: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. RESULTS: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p = .043 and .033). The SMWR was significantly higher in the Study Group at 12 weeks (p = .0207). CONCLUSIONS: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Ciático / Trasplante de Tejidos / Técnicas de Sutura / Traumatismos de los Nervios Periféricos / Regeneración Nerviosa Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Acta Chir Belg Año: 2018 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Ciático / Trasplante de Tejidos / Técnicas de Sutura / Traumatismos de los Nervios Periféricos / Regeneración Nerviosa Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Acta Chir Belg Año: 2018 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido