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Enhancement of antiviral capacity of transgenic silkworms against cytoplasmic polyhedrosis virus via knockdown of multiple viral genes.
Jiang, Liang; Peng, Zhengwen; Guo, Huizhen; Sun, Jingchen; Sun, Qiang; Xia, Fei; Huang, Chunlin; Xu, Guowen; Xia, Qingyou.
Afiliación
  • Jiang L; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Peng Z; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Guo H; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Sun J; College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
  • Sun Q; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Xia F; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Huang C; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Xu G; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
  • Xia Q; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China. Electronic address: xiaqy@swu.edu.cn.
Dev Comp Immunol ; 77: 138-140, 2017 12.
Article en En | MEDLINE | ID: mdl-28735962
Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), a major pathogen of silkworms, causes serious economic losses in sericulture. The BmCPV genome contains 10 discrete dsRNA segments; among these, S1, S2, S3, S4, S6, and S7 encode virus structural proteins, whereas S5, S8, S9, and S10 encode nonstructural proteins. In an attempt to create an anti-BmCPV silkworm strain, we constructed transgenic RNAi vector pb-CNS for knockdown of S5, S8, S9, and S10, and pb-SNS targeting S1, S2, S4, S5, and S8. Transgenic silkworm line CNS and SNS were generated via microinjection of the practical diapause silkworm strain Furong. Following infection via the oral administration of a high dose of BmCPV, the mortality rates of the nontransgenic control, CNS, and SNS were 91%, 37%, and 41%, respectively. qPCR showed that the viral mRNA content in CNS and SNS was significantly lower than that in the nontransgenic line. The economic traits of CNS and SNS were not affected. These results suggest that the knockdown of multiple BmCPV genes significantly enhances the antiviral capacity of the silkworm.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reoviridae / Bombyx / Proteínas Virales / Infecciones por Reoviridae / ARN Interferente Pequeño Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reoviridae / Bombyx / Proteínas Virales / Infecciones por Reoviridae / ARN Interferente Pequeño Límite: Animals Idioma: En Revista: Dev Comp Immunol Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos